Sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson)

A technology for juvenile sex and yellow catfish, which is applied to fish sex identification, quickly and accurately distinguishes the sex of hybrid yellow catfish [Yellow catfish╳Washer's yellow catfish] juveniles, common yellow catfish and "Quanxiong No. 1" "The field of gender identification of juvenile yellow catfish can solve the problems of high equipment requirements, high detection costs, and cumbersome method steps, and achieve the effect of increasing benefits

CN103529036AInactive Publication Date: 2014-01-22NANJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-01-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a sex identification method for juvenile fish of hybrid pelteobagrus fulvidraco (richardson) [pelteobagrus fulvidraco (richardson) (male)* pelteobagrus vachelli (richardson) (female)]. The hybrid pelteobagrus fulvidraco (richardson) having an individual body length of above 3 cm is identified by utilization of a method of living-body gonad squashing, acetocarmine staining and ethanol crystal violet staining, and the accuracy of the method is verified by a paraffin section of the gonad tissue. The sex identification method is suitable for sex identification for juvenile fish of common pelteobagrus fulvidraco (richardson) and of "all-male No.1" pelteobagrus fulvidraco (richardson). By the sex identification method, sex identification of one juvenile fish can be completed in 3 min, and 100 fishes can be identified by cooperation of two persons for 2 h. The sex identification method has characteristics of high efficiency, economy, rapidness, accuracy and stability. The sex identification method has important application value for male fry cultivation of the pelteobagrus fulvidraco (richardson) and the hybrid pelteobagrus fulvidraco (richardson) thereof, and can be applied for rapid sex identification of a pelteobagrus fulvidraco (richardson) commodity summer fry.
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Description

technical field

[0001] The invention belongs to fish gender identification technology in the technical field of aquatic organisms. More specifically, the invention relates to a method for quickly and accurately identifying hybrid yellow catfish [yellow catfish (♀)╳Vascher's yellow catfish (♂)] juveniles This method is also applicable to the sex identification of common yellow catfish and "Quanxiong No. 1" yellow catfish juveniles. The invention has important application value in cultivating male seedlings of the yellow catfish and its hybrid yellow catfish, and can be applied to the rapid sex identification of commercial yellow catfish seedlings. Background technique

[0002] At present, there are mainly two species of yellow catfish cultured, namely, hybrid yellow catfish [yellow catfish (♀)╳Washer's yellow catfish (♂)] and "Quanxiong No. 1" yellow catfish.

[0003] Both pelteobagrus fulvidraco (Richardson) and Pelteobagrus vachelli (Richardson) belong to Siluriformes, Bag...

Examples

specific example

[0022] 1. Preliminary preparation for identification

[0023] 1) Drugs: 5% acetic acid solution, acetic acid (glacial acetic acid), magenta stain (phenol red), crystal violet stain, ammonium oxalate, 95% ethanol, double distilled water

[0024] 2) Equipment: biological microscope, 1mL syringe, ophthalmic scissors, iris tweezers, glass slide, cover glass, dropper

[0025] 3) Preparation of staining solution: Prepare glacial acetic acid carmine solution and ethanol crystal violet solution according to the method introduced in the manual

[0026] 2. Gender identification:

[0027] The Laboratory of Aquatic Economic Animal Germplasm Resources and Genetic Breeding, School of Life Sciences, Nanjing Normal University, Jiangsu Province, artificially bred hybrid yellow catfish in the Lukou Yellow Catfish Experimental Base in June 2013. Fish (♂)] 100,000 tails, 20,000 ordinary yellow catfish, and 50,000 "Quanxiong No. 1" yellow catfish. 30 days after the opening of the hybrid yellow ...