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Method for extracting and separating nucleoside from rape pollen and rape bee pollen and application thereof in resisting benign prostatic hyperplasia

A technology for rape bee pollen and prostatic hyperplasia, applied in the food field, can solve the problems of restricting the development and utilization of rape pollen and rape bee pollen, unclear and the like

Inactive Publication Date: 2014-01-29
徐一达
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reports on the chemical constituents in rapeseed pollen and rapeseed bee pollen, but before the present invention, which composition is the functional ingredient for inhibiting benign prostatic hyperplasia and anti-prostatitis is not clear, which severely limits the use of rapeseed pollen and rapeseed bee pollen. develop and use

Method used

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  • Method for extracting and separating nucleoside from rape pollen and rape bee pollen and application thereof in resisting benign prostatic hyperplasia
  • Method for extracting and separating nucleoside from rape pollen and rape bee pollen and application thereof in resisting benign prostatic hyperplasia
  • Method for extracting and separating nucleoside from rape pollen and rape bee pollen and application thereof in resisting benign prostatic hyperplasia

Examples

Experimental program
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Embodiment 1

[0019] Take 5kg of rapeseed bee pollen, crush it, pass through 60 mesh, take the under-sieve powder, crush the over-sieve again through 60 mesh, take the under-sieve powder, place the under-sieve powder in a 50-liter extraction tank, add 40 liters of 70 % ethanol, extract at room temperature for 3 hours, filter, repeat the extraction of residue once more, filter, take filter residue and place it in a 100-liter extraction tank, then add 50 liters of deionized water, extract for 3 hours at 60 ° C, filter, and take the filtrate to obtain a filtrate 49.8 liters, concentrated under reduced pressure to 5 liters, then passed through the macroporous resin column AB-8 (10 × 150cm) column, first eluted with 20 liters of deionized water, then eluted with 10 liters of 5% ethanol, and then washed with 20 liters of 10 % ethanol and then 90% ethanol, the 10% ethanol eluate was collected and concentrated under reduced pressure to obtain about 2 liters of concentrate. Put 2 liters of macroporo...

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Abstract

The invention relates to the technical field of food, healthcare food and medicine, and particularly relates to a method for extracting and separating nucleoside from rape pollen and rape bee pollen and application thereof in resisting benign prostatic hyperplasia. Total nucleosides of the rape pollen and rape bee pollen are obtained through water extraction, macroporous resin column separation and MCI column chromatography purification; according to the model checking on the mouse benign prostatic hyperplasia caused by testosterone propionate, the total nucleosides have a remarkable effect in inhibiting benign prostatic hyperplasia. According to the method provided by the invention, a material basis for resisting benign prostatic hyperplasia in the water extracts of rape pollen and rape bee pollen is determined, and the method is of important value for deep development of the rape pollen and rape bee pollen.

Description

technical field [0001] The invention relates to the technical fields of food, health food and medicine, and refers to a method for extracting and separating nucleosides from rapeseed pollen and rapeseed pollen and its anti-prostatic hyperplasia application. Background technique [0002] Rapeseed pollen is the pollen of the oilseed rape (Brassica campestris L.), and bee pollen is the pollen ball collected by bees from the anthers of the rapeseed plant. A large number of literatures have reported that rape pollen and rape bee pollen have the effects of delaying aging, lowering serum cholesterol, improving immunity, inhibiting benign prostatic hyperplasia and anti-prostatitis, especially for benign prostatic hyperplasia and prostatitis. There are many reports on the chemical constituents in rapeseed pollen and rapeseed bee pollen, but before the present invention, which composition is the functional ingredient for inhibiting benign prostatic hyperplasia and anti-prostatitis is ...

Claims

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Application Information

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IPC IPC(8): A61K36/31A61K31/708A61P13/08A23L1/30A61K31/7076A61K31/7072A61K133/00A23L33/10
Inventor 徐一达
Owner 徐一达
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