37 results about "Propionate testosterone" patented technology

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g / mu l and an adjuvant concentration of 50-150 mu g / mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

The invention discloses a methandienone monoclonal antibody and a preparation method and application thereof, belonging to the field of medical immunology detection. The methandienone monoclonal antibody disclosed by the invention is secreted by the hybridoma cell strain of the mouse monoclonal antibody of which the preservation number is CGMCC No.3807. A complete antigen with immunogenicity and an envelope antigen for ELISA are prepared by coupling a certain amount of methandienone molecules on different protein carriers; and the complete antigen is used for immunizing the Balb / C mice to obtain the hybridoma cell strain which can stably secrete DMT monoclonal antibodies and has high affinity and specificity through cell fusion, indirect ELISA screening and subcloning purification culture. The monoclonal antibody of the invention has higher affinity and sensitivity to DMT and has obvious specificity, and the cross reaction rate of the monoclonal antibody with analog testosterone propionate esters and trenbolone is lower than 1%. The invention can be applied to rapid detection of methandienone.

The invention discloses an application of gallocatechin in the preparation of drugs for resisting benign prostatic hyperplasia. According to the invention, through a prostatic hyperplasia model in ovariectomized rats induced by testosterone propionate, gallocatechin is selected as a therapeutic drug so as to observe the influence on rat prostate weight and index and pathological changes of prostate tissues. Research results show that gallocatechin can significantly reduce the prostate weight and prostate index of the animal model and improve pathological symptoms of prostate tissues.

The invention relates to the technical field of food, healthcare food and medicine, and particularly relates to a method for extracting and separating nucleoside from rape pollen and rape bee pollen and application thereof in resisting benign prostatic hyperplasia. Total nucleosides of the rape pollen and rape bee pollen are obtained through water extraction, macroporous resin column separation and MCI column chromatography purification; according to the model checking on the mouse benign prostatic hyperplasia caused by testosterone propionate, the total nucleosides have a remarkable effect in inhibiting benign prostatic hyperplasia. According to the method provided by the invention, a material basis for resisting benign prostatic hyperplasia in the water extracts of rape pollen and rape bee pollen is determined, and the method is of important value for deep development of the rape pollen and rape bee pollen.

The invention provides a method for measuring sex hormone residue in cosmetics. An analysis method for simultaneously measuring pregnendione, testosterone propionate, medroxyprogesterone acetate, norgestrelm, hydroxyprogesterone acetate and methyltestosterone residue in the cosmetics through high-performance liquid chromatography-tandem mass spectrum is established. The method comprises the following steps: firstly pretreating cosmetic samples of different preparations, and taking acetonitrile and n-hexane as extraction solvents, ultrasonically extracting for 20min, and centrifuging a sample extracting solution for 10min in the speed of 12000r / min; selecting the acetonitrile and water as moving phases to perform isogradient elution, wherein the flow velocity is 0.3mL / min, and the column temperature is 25 DEG C, and performing separation by adopting an octadecyl silane chemically bonded silica chromatographic column. A result shows that a linear regression equation correlation coefficient R of each of six sex hormones is 0.9993-0.99989, the recycling rate is between 97.6%-99.5%, and the accuracy is between 0.5%-3.1%. The method provided by the invention is simple, sensitive, fast, reliable, good in reproducibility, and suitable for content analysis detection of the hormone addition of sex hormones in the cosmetics.

The invention discloses a clean production and preparation method of testosterone propionate. According to the method, testosterone is used as a raw material and subjected to esterification and refining so as to obtain a testosterone propionate product with a purity of greater than or equal to 99.5%; and waste acid water generated in the preparation process is subjected to neutralization with sodium carbonate, decoloration with activated carbon, suction filtration and concentration so as to prepare a sodium propionate byproduct, so pollution of wastewater to the environment is reduced. According to the invention, when high-purity testosterone propionate is prepared, the components in the waste acid water are effectively recycled to obtain the byproduct sodium propionate, so cost is reduced, and the clean production of testosterone propionate is realized.

The present invention is about a method of producing testosterone formulation and the testosterone formulation produced thereby. The method of this invention comprises dissolving testosterone propionate and dibucaine HCl in alcohol, adding particular percentages of polyethylene glycol 400 and polyethylene glycol 4000, and cooling under particular speed to produce the testosterone formulation which has the advantages of moderate viscosity, easy to use and excellent particle consistency.

The invention discloses application of gallocatechin gallate in preparation of a benign prostatic hyperplasia resistant medicament. Influence on prostate weight and prostate indexes of a rat and pathological change of prostate tissues are observed through a testosterone propionate induced castrated rat prostatic hyperplasia model by using the gallocatechin gallate as a treatment medicament. Research results show that the gallocatechin gallate can remarkably reduce the prostate weight and the prostate index of a model animal and improve the pathological symptoms of the prostate tissues.

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g / mu l and an adjuvant concentration of 50-150 mu g / mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

The invention discloses a pharmaceutical composition against benign prostatic hyperplasia. The composition is a preparation prepared from the following raw material medicines in parts by weight: 20-60parts of all-grass of thellung pimpinella, 10-40 parts of kudzuvine roots, 5-20 parts of hawthorn fruits, 5-20 parts of Chinese wolfberry fruits, and 1-4 parts of American ginseng. The pharmaceuticalcomposition of the present invention is useful for treating benign prostatic hyperplasia with definite effect. Experiments on a rat prostatic hyperplasia model find that the pharmaceutical composition of the invention can improve the body hormone endocrine disorder of the animal model, enhance the antioxidant capacity of the animal model, and have a good therapeutic effect on benign prostatic hyperplasia induced by testosterone propionate.

The invention discloses application of Wubi Chinese yam pills in preparation of a medicine for treating benign prostatic hyperplasia. The Wubi Chinese yam pills consist of Chinese yams, cistanche deserticola, schisandra chinensis, semen cuscutae, eucommia ulmoides, achyranthes bidentata, rhizoma alismatis, prepared rehmannia roots, dogwood, poria cocos, morinda officinalis and red halloysite. According to the invention, testosterone propionate is used to induce benign prostatic hyperplasia modeling of Wistar rats; continuously intragastric administration of the Wubi Chinese yam pills is performed for 12 weeks; it is found that the Wubi Chinese yam pills have obviously improved pathological changes on BPH rats, the Wubi Chinese yam pills are related to the expression of AR and PSA in prostate tissue at the mRNA level which can be adjusted by the Wubi Chinese yam pills, and the treatment effect of the Wubi Chinese yam pills in a medium-dose group is especially good, and significant differences (p is smaller than 0.05) exist; it is indicated that the Wubi Chinese yam pill has a certain treatment or improvement effect on BPH rats, so that the Wubi Chinese yam pill is used for treatingbenign prostatic hyperplasia diseases, and good news is brought to BPH patients.

The invention discloses clean itching relieving paste for treating non-neoplastic epithelial disorders of the vulva, belonging to the field of traditional Chinese medicine preparations. The non-neoplastic epithelial disorders of the vulva refers to a group of chronic diseases that the female vulva skin and mucous membrane tissue suffer degeneration and pigment change, and is most clinically manifested on the middle-aged women younger than 50 years old. The traditional external ointment realizes relatively slow cure and bad effect. The clean itching relieving paste disclosed by the invention is characterized by being prepared by taking the Chinese and western medicines of vitamin B1, vitamin B2, vitamin E, diethylstilbestrol, minium, clam meal and borneol as the major raw materials and taking fluocinolone acetonide ointment or testosterone propionate ointment as an auxiliary. The product is pink emulsifiable paste, and has the functions of reliving itching and coloring, softening cutin, removing necrotic tissue and promoting granulation and relieving swelling and pain; the paste can quickly permeate into the skin surface, has short course of treatment, realizes quick cure, and does not cause recurrence easily while preventing allergy and toxic side effect; and the cure cost is relatively low. The clean itching relieving paste disclosed by the invention has a simple structure.

The invention provides a preparation method of testosterone propionate intermediate 17 beta-hydroxyandrost-4-en-3-one (testosterone). With 4-androstenedione as the raw material, a 3-keto protection reaction, a 17-keto reduction reaction and a 3-keto deprotection reaction are sequentially conducted to prepare testosterone propionate intermediate 17 beta-hydroxyandrost-4-en-3-one (testosterone). The technology has the advantages that reaction selectivity is high, operation is easy and convenient, industrialization is easy to achieve, the production cost is low, and the yield is high.

The invention belongs to the field of medicines, and discloses novel application of a mango aglycone derivative. An in-vitro test shows that the mango aglycone derivative can obviously inhibit activity of 5 alpha-reductase, can remarkably lower prostate wet weight index of a testosterone propionate replication prostate hyperplasia model mouse, can lower a ratio of E2 / T in blood serum, and can lower activity of the 5 alpha-reductase in a liver tissue, so that the mango aglycone derivative is indicated to have effect in inhibiting activity of 5 alpha-reductase, can be used for treating 5 alpha-reductase expression or superhigh-activity-associated diseases, for example testosterone propionate; moreover, the mouse stomach is filled with the drug in maximum administration dosage of 21.6g / kg, and therefore, the drug has very good clinical application prospect.

The invention discloses a methandienone monoclonal antibody and a preparation method and application thereof, belonging to the field of medical immunology detection. The methandienone monoclonal antibody disclosed by the invention is secreted by the hybridoma cell strain of the mouse monoclonal antibody of which the preservation number is CGMCC No.3807. A complete antigen with immunogenicity and an envelope antigen for ELISA are prepared by coupling a certain amount of methandienone molecules on different protein carriers; and the complete antigen is used for immunizing the Balb / C mice to obtain the hybridoma cell strain which can stably secrete DMT monoclonal antibodies and has high affinity and specificity through cell fusion, indirect ELISA screening and subcloning purification culture. The monoclonal antibody of the invention has higher affinity and sensitivity to DMT and has obvious specificity, and the cross reaction rate of the monoclonal antibody with analog testosterone propionate esters and trenbolone is lower than 1%. The invention can be applied to rapid detection of methandienone.

The invention provides a method for preventing and treating aspermia and necrospermia of a breeding boar. The method employs a combination of a traditional Chinese medicine therapy and a western medical therapy. According to the western medical therapy, 100 to 150 mg of a testosterone propionate injection is used for cervical intramuscular injection once every other day, and altogether four injection operations are carried out; and three to five vitamin C capsules and two to three vitamin AD capsules are simultaneously fed with a feed each time, and the capsules are fed for a consecutive period of seven days. According to the traditional Chinese medicine therapy, traditional Chinese medicinal raw materials are mixed and powdered and then orally taken by the breeding boar, wherein the traditional Chinese medicinal raw materials consist of, by weight, 25 to 35 parts of dodder seeds, 25 to 35 parts of Chinese magnoliavine fruit, 25 to 35 parts of wolfberries, 25 to 35 parts of raspberries, 45 to 55 parts of Herba Epimedii, 45 to 55 parts of eucommia bark and 20 to 30 parts of cassia bark; one dosage of the powdered traditional Chinese medicinal raw materials is taken each day and taken twice each day; and the powdered traditional Chinese medicinal raw materials are taken for consecutive period of five days. With the method provided by the invention, breeding boars with aspermia and necrospermia have more sensitive visual sense and hearing and are fond of standing and moving; little foam saliva flows out from the mouths of the breeding boars; normally after five days, the breeding boars have good appetite, improved sexual desire and a body temperature increase of about 0.3 DEG C, and the result of microscopic examination of seminal fluids shows that a great number of sperms are moving; and normally, aspermia and necrospermia can be thoroughly treated after continuous treatment for 7 to 10 days.

The invention discloses a preparation method of testosterone propionate, which belongs to the technical field of preparation and processing of medicines. In the method, testosterone is used as a raw material, and through the catalysis of a catalyst, an organic base is used as an acid-binding agent to undergo esterification reaction with propionic anhydride to obtain testosterone propionate. The method of the present invention avoids the use of expensive and highly toxic acid chlorides, and the solvent used is water-insoluble, so after the reaction is completed, the product can be directly layered and extracted without adding water to separate out the product, reducing the consumption of water, and the solvent can be Recycling and reuse makes the whole process more environmentally friendly; the reaction product of the invention has high purity, easy purification, product quality and yield higher than 110%, is suitable for industrialized large-scale production, and has good economic benefits.

The invention discloses a preparation method of testosterone propionate, and belongs to the technical field of medicine preparation and processing. According to the method, testosterone is used as a raw material, under the catalytic action of a catalyst, organic alkali is used as an acid-binding agent to be subjected to esterification reaction with propionic anhydride, and testosterone propionateis obtained. In the invention, the use of expensive and toxic acyl chloride is avoided, and the used solvent is non-water-soluble, so that after the reaction is finished, the product can be directly extracted in a layered manner, water does not need to be added to separate out the product, the use amount of water is reduced, the solvent can be recycled, and the whole process is more environment-friendly; in addition, the reaction product is high in purity and easy to purify, the product quality yield is higher than 110%, and the method is suitable for industrial large-scale production and hasgood economic benefits.

The invention discloses application of trigonelline in preparation of a medicine for preventing or treating prostatic hyperplasia. A rat benign prostatic hyperplasia (BPH) model is established through a testosterone propionate induction method, and a method of histology and molecular biology is adopted to verify that the trigonelline can significantly reduce the prostate wet weight and reduce the prostate index; the prostatic hyperplasia is observed to be obviously relieved in form; the PSA level in serum is obviously reduced; and it shows that the trigonelline has an improvement effect on BPH of rats and can be used for preparing the medicine for preventing or treating the prostatic hyperplasia.

The invention discloses the application of testosterone propionate as a medicine for constructing an animal model with dysspermia. An animal experiment shows that: the spermatogenesis of an experimental animal can be effectively suppressed and the quality and activity of sperm are reduced after the in vitro injection of the testosterone propionate, and semen routines (the count, quality and activity of the sperm) of the experimental animal cannot be restored after the in vitro injection of the testosterone propionate is stopped, so the process is irreversible. The experimental result shows that: the testosterone propionate serving as the medicine can be used for constructing the animal model with the dysspermia.

The invention discloses the application of testosterone propionate as a medicine for constructing an animal model with dysspermia. An animal experiment shows that: the spermatogenesis of an experimental animal can be effectively suppressed and the quality and activity of sperm are reduced after the in vitro injection of the testosterone propionate, and semen routines (the count, quality and activity of the sperm) of the experimental animal cannot be restored after the in vitro injection of the testosterone propionate is stopped, so the process is irreversible. The experimental result shows that: the testosterone propionate serving as the medicine can be used for constructing the animal model with the dysspermia.

The invention relates to an extraction and detection method for hormone substances in Antarctic krill. The method comprises the steps of refrigerating and drying the Antarctic krill, grinding and crushing Antarctic krill after refrigerating and drying; extracting the crushed and ground Antarctic krill by using ethyl acetate, thus acquiring first extracting solution; extracting the first extractingsolution by using sodium hydroxide buffer solution, thus acquiring second extraction solution; edulcorating and purifying the second extraction solution by using a QuEChERs solid phase extraction column, separating after purification, and taking supernate; and dehydrating the supernate, then redissolving by using the ethyl acetate, filtering by a membrane, thus acquiring extractives including cortisone, testosterone propionate, megestrol acetate, hydroxyprogesterone caproate, cortisone acetate, hexadecadrol and nandrolone. The extraction method provided by the invention is simple in operationprocess, short in consumed time, and capable of fully removing purities.

The invention discloses a novel medicament for penis built-in pills, a preparation method and a medicament placing device thereof. The novel medicament comprises a tamsulosin urethral water flooding pill, a sarcolysin penis built-in dropping pill and a testosterone propionate urethral lipid pill. When the medicament placing device is used, firstly, the penis is conventionally disinfected, the outer side of a medicament injection hose of the medicament placing device filled with pills is coated with glycerinum for lubrication, the medicament injection hose is inserted into the penis urethra, amedicament pushing rod is pushed forwards, and a corresponding number of pills are injected according to the required injection dosage. The administration mode and the administration path are changed,and rear-end medication of liver and kidney metabolism is adopted. In the treatment and health care process of men, a target organ can be used for direct administration, absorption and effect taking,the medicament composition is small, the medicament concentration in local blood is high, the medicament effect is strong, the medicament can take effect while being absorbed during urethra administration, the non-absorbed medicament is excreted in a harmless manner, and the medicament is a safe and efficient novel administration way, a novel medication mode and a novel medicament, and has a relatively high market prospect and a relatively strong medicament research and development prospect.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating androgenetic alopecia and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the raw material medicines in parts by weight: 10-30 parts of fructus viticis, 15-30 parts of cacumen platycladi, 10-15 parts of cortex phellodendri, 15-30 parts of herba ecliptae, 20-30 parts of fructus lycii, 10-20 parts of ligusticum wallichii, 8-15 parts of radix angelicae and 5-15 parts of fructus psoraleae. The preparation method and application of the traditional Chinese medicine composition for treating androgenetic alopecia are further provided. Testosterone propionate induced mouse androgenic alopecia model animal experiments show that the traditional Chinese medicine composition can promote the hair growth of a testosterone propionate induced androgenic alopecia mouse, improve the tissue form of skin lesion and inhibit the synthesis of type II 5 alpha reductase in the skin lesion. Therefore, the traditional Chinese medicine composition has a relatively good treatment effect on androgenetic alopecia.

The invention discloses a method for inducing moschus berezovskii to secrete musk for many times in one year by utilizing exogenous hormone, and belongs to the field of moschus berezovskii breeding. The method comprises the following steps of performing intramuscular injection on male moschus berezovskii for the first time by adopting a testosterone propionate injection, gradually increasing the testosterone propionate injection every day for re-injection, and stopping the injection until enabling the male moschus berezovskii to have a musk secretion reaction; and finishing inducing the moschus berezovskii to secrete the musk. According to the method, the annual yield and quality of the musk are effectively improved, theoretically, the moschus berezovskii can secrete the musk for four times in a year to the maximum extent, and musk production and sustainable utilization of musk resources are promoted.

The invention belongs to the field of medicines, and discloses novel application of a mango aglycone derivative. An in-vitro test shows that the mango aglycone derivative can obviously inhibit activity of 5 alpha-reductase, can remarkably lower prostate wet weight index of a testosterone propionate replication prostate hyperplasia model mouse, can lower a ratio of E2 / T in blood serum, and can lower activity of the 5 alpha-reductase in a liver tissue, so that the mango aglycone derivative is indicated to have effect in inhibiting activity of 5 alpha-reductase, can be used for treating 5 alpha-reductase expression or superhigh-activity-associated diseases, for example testosterone propionate; moreover, the mouse stomach is filled with the drug in maximum administration dosage of 21.6g / kg, and therefore, the drug has very good clinical application prospect.

The invention provides a benign prostatic hyperplasia resisting urtica hyperborean Jacq extract, a preparation method, and an application of the benign prostatic hyperplasia resisting urtica hyperborean Jacq extract. The preparation method comprises the following steps of (1) drying urtica hyperborean Jacq, performing crushing, performing extraction with 95% alcohol, performing extraction with 50%alcohol, obtaining 95% alcohol extractum, 50% alcohol extractum and aqueous extractum; (2) removing the alcohol from the urtica hyperborean Jacq extracting solution, and firstly performing degreasingwith petroleum ether to obtain a degreased extract; and (3) extracting the degreased extract with ethyl acetate, collecting part of the ethyl acetate, performing concentration, and preforming dryingto obtain the urtica hyperborean Jacq extract. The urtica hyperborean Jacq and ethyl acetate extraction positions and alcohol dissolving positions have good treatment effects on model mice suffering from benign prostatic hyperplasia, induced with testosterone propionate.

The invention relates to the technical field of medicines, in particular to application of halcinonide and a derivative thereof in preparation of a medicine for treating and / or preventing cerebrovascular diseases. The halcinonide and the derivative thereof have a structural general formula shown in formula (I), and the treatment effect of the halcinonide and the derivative thereof on ischemic cerebral stroke is evaluated through an MCAO cerebral ischemia-reperfusion injury animal model. Experimental results show that the halcinonide and the derivatives thereof prednisone and testosterone propionate can significantly improve the score of animal neurological impairment and reduce the area of infarct focus, can be used for treating ischemic stroke, and have good clinical application prospects.

The invention discloses a clean production and preparation method of testosterone propionate. The method uses testosterone as a raw material, undergoes esterification and refining to obtain testosterone propionate with a product purity ≥ 99.5%, and the waste acid water produced in the preparation process is passed through sodium carbonate Neutralization, activated carbon decolorization, suction filtration and concentration, the by-product sodium propionate is prepared, which reduces the pollution of waste water to the environment. While preparing high-purity testosterone propionate, the present invention effectively recycles components in waste acid water to obtain by-product sodium propionate, thereby reducing costs and realizing clean production of testosterone propionate.