System and method for measuring electrical characteristics of biological cells through nano-electrode array under physiological conditions

A technology of nano-electrode arrays and physiological conditions, which is applied in the field of systems for measuring electrical properties of biological cells, can solve problems such as difficult operation, limited range of samples to be detected, and cells affecting the authenticity of measured data.

Active Publication Date: 2014-02-05
CHANGCHUN UNIV OF SCI & TECH
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Problems solved by technology

However, this technique requires the process of adsorbing cell membranes through microelectrodes, which is extremely difficult to operate, requires long-term training for experimenters, and also severely limits the range of samples tested by this technique. The process may seriously damage cells. The authenticity of the measured data

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  • System and method for measuring electrical characteristics of biological cells through nano-electrode array under physiological conditions
  • System and method for measuring electrical characteristics of biological cells through nano-electrode array under physiological conditions
  • System and method for measuring electrical characteristics of biological cells through nano-electrode array under physiological conditions

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Embodiment Construction

[0020] Such as figure 1 , 4 As shown, the system for measuring the electrical characteristics of biological cells with a nanoelectrode array under physiological conditions of the present invention includes: a nanoelectrode array 1, an inverted or upright optical microscope 3, a first probe control module 4, a second probe control module 5 and Electrical signal processing module 7. The measured object 2 is placed on the nano-electrode array 1, the first probe control module 4 and the second probe control module 5 are respectively placed on both sides of the inverted or upright optical microscope 3, and the first probe control module 4 and the The second probe control module 5 is placed opposite to each other on the same horizontal plane, and the actions and operations of the first probe control module 4 and the second probe control module 5 are respectively controlled by a computer. First, use the first probe to scan the sample to be tested, determine the position of the obje...

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Abstract

The invention relates to a system and method for measuring electrical characteristics of biological cells through a nano-electrode array under physiological conditions. The system consists of the nano-electrode array, an inverted or upright optical microscope, a probe control module and an electrical signal processing module. The system is arranged in a culture environment suitable for cell in-vitro growth, the electrical characteristics of a single cell are detected through the nano-electrode array and probes, the strength of an electrical signal is measured and recorded according to selected points or areas, and the electrical characteristics of a cell body are obtained on a nano-scale. The invention aims to improve an existing micro-electrode-array-based method for measuring the electrification amount of the biological cells by using the nano-electrode array so as to provide the method and system capable of measuring the electrification amount of the biological cells, applying an electric excitation signal to the single cell for observing the change of the single cell and also detecting the electrical characteristics of the cell by utilizing the probes.

Description

technical field [0001] The invention relates to a system and a method for measuring electrical properties of biological cells, in particular to a system and a method for measuring electrical properties of biological cells with a nanometer electrode array under physiological conditions. Background technique [0002] At present, the most commonly used method for measuring the electrical characteristics of biological cells is the patch clamp technique. The patch clamp technique uses a glass microelectrode pipette to attract the cell membrane with only 1-3 ion channels and an area of ​​several square microns through negative pressure. Sealed up, this current intensity is measured with an extremely sensitive current monitor (patch clamp amplifier), representing a single ion channel current. However, this technique requires the process of adsorbing cell membranes through microelectrodes, which is extremely difficult to operate, requires long-term training for experimenters, and al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 王作斌刘兰娇于占江娄俊翁占坤宋正勋刘妍许红梅胡贞王庆康谢晖杨宝学
Owner CHANGCHUN UNIV OF SCI & TECH
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