A porcine chimeric antibody against Haemophilus parasuis and its construction method and application

A technology of Haemophilus suis and chimeric antibodies, applied in the field of biomedicine, can solve problems such as the ability of chimeric antibodies to resist virus attacks that have not been mentioned, and achieve the effect of antibacterial effect

Inactive Publication Date: 2015-08-12
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are only reports of murine-pig chimeric antibodies constructed against porcine reproductive and respiratory syndrome virus and porcin...

Method used

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  • A porcine chimeric antibody against Haemophilus parasuis and its construction method and application
  • A porcine chimeric antibody against Haemophilus parasuis and its construction method and application
  • A porcine chimeric antibody against Haemophilus parasuis and its construction method and application

Examples

Experimental program
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Embodiment 1

[0041] Example 1 Amplification of Monoclonal Antibody Light and Heavy Chain Variable Region Sequence and Pig Antibody Light and Heavy Chain Constant Region

[0042] 1) Amplification of light chain variable region and heavy chain variable region sequence of mouse anti-Haemophilus parasuis monoclonal antibody

[0043]For the preparation and identification of anti-Haemophilus parasuis (HPS) monoclonal antibody 1D8, please refer to the literature: Screening of monoclonal antibodies with protective efficacy and identification of immunogenic proteins against Haemophilus parasuis, Tian Huabin, "Chinese Agriculture Academy of Sciences, 2012. It was isolated and preserved by the Porcine Disease Molecular Diagnosis Group of the Porcine Infectious Diseases Department of Harbin Veterinary Research Institute of Chinese Agricultural Science Research.

[0044] After identifying the subtype of the antibody, the monoclonal antibody cells were subjected to total RNA extraction (according to th...

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Abstract

The invention discloses an anti-Haemophilus parasuis porcine chimeric antibody, and a construction method and application thereof. The porcine chimeric antibody is formed by connecting a light chain and a heavy chain through a disulfide bond and a non-covalent bond, wherein the light chain sequentially comprises a murine anti-Haemophilus parasuis monoclonal antibody light chain variable region and a porcine IgG light chain constant region from N end to C end, and the heavy chain sequentially comprises a murine anti-Haemophilus parasuis monoclonal antibody heavy chain variable region and a porcine IgG heavy chain constant region. The experiment proves that the anti-Haemophilus parasuis porcine chimeric antibody has obvious antibacterial effect in vivo and in vitro, and therefore, has wide application prospects in detecting or preventing Haemophilus parasuis infection.

Description

technical field [0001] The invention relates to a porcine chimeric antibody and its construction method and application, in particular to a porcine chimeric antibody capable of resisting Haemophilus parasuis and its construction method and application, belonging to the technical field of biomedicine. Background technique [0002] Haemophilus parasuis (HPS) is a non-hemolytic, NAD-dependent, Gram-negative bacterium belonging to the family Pasteurellaceae. Haemophilus parasuis is an important pathogen of the upper respiratory tract of pigs and the pathogen of Glasser's Disease. The disease is characterized by meningitis, arthritis, and fibrinous polyserositis, sometimes with sepsis and acute pneumonia. In recent years, with the change of pig breeding methods, such as the early weaning program and the application of the three-point breeding system, the morbidity and mortality of pigs caused by Haemophilus parasuis have increased year by year, and it has become a threat to comm...

Claims

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Application Information

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IPC IPC(8): C07K16/12C12N15/13C12N15/866G01N33/569A61K39/40A61P31/04
Inventor 李曦符芳柴政田华彬
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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