Multi-template monodisperse active notoginsenoside molecular imprinted polymer and preparation method thereof

A molecular imprinting and template molecule technology, applied in the field of bioengineering, to achieve the effects of reduced damage, uniform particle size and high yield

Inactive Publication Date: 2014-02-12
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of multi-template molecularly imprinted polymers to separate and pur

Method used

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  • Multi-template monodisperse active notoginsenoside molecular imprinted polymer and preparation method thereof
  • Multi-template monodisperse active notoginsenoside molecular imprinted polymer and preparation method thereof
  • Multi-template monodisperse active notoginsenoside molecular imprinted polymer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 348.5mg ginsenoside Rb 1 , Ginsenoside Rg 1 and notoginsenoside R 1 The mixture (the molar ratio of the three is 44:43:13) and 1.00mmol functional monomer MAA were dissolved in 25mL DMF solvent, dissolved by ultrasonication for 10min, and pre-polymerized for 2-4h. Then add 6.00 mmol of cross-linking agent EGDMA and 48.72 mg of initiator AIBN, and dissolve by ultrasonication for 10 min. The mixed solution was blown with nitrogen gas for 10 minutes, heated to 60° C. on a constant temperature shaker, and polymerized at 300 rpm for 24 hours.

[0036] Collect by centrifugation and elute with methanol-glacial acetic acid (v / v, 9:1) to remove template molecules until no template molecules can be detected at 204nm by UV spectrophotometer, then wash with methanol until neutral, and then 45°C After vacuum drying for 12 hours, the multi-template monodisperse notoginseng active saponin molecularly imprinted polymer (MIP-1) was obtained.

[0037] No template molecule notoginseng...

Embodiment 2

[0039] 348.5mg ginsenoside Rb 1 , Ginsenoside Rg 1 and notoginsenoside R 1 The mixture (the molar ratio of the three is 44:43:13) and 1.50mmol functional monomer AU were dissolved in 30mL THF solvent, dissolved by ultrasonication for 10min, and pre-polymerized for 2-4h. Then add 6.00 mmol of cross-linking agent TRIM and 75.88 mg of initiator AIBN, and dissolve by ultrasonication for 5 min. The mixed solution was blown with nitrogen gas for 10 minutes, heated to 50° C. on a constant temperature shaker, and polymerized at 200 rpm for 36 hours.

[0040] Collect by centrifugation and elute with methanol-glacial acetic acid (v / v, 9:1) to remove template molecules until no template molecules can be detected at 204nm by UV spectrophotometer, then wash with methanol until neutral, and then 45°C After vacuum drying for 24 hours, the multi-template monodisperse notoginseng active saponin molecularly imprinted polymer (MIP-2) was obtained.

[0041] No template molecule notoginseng sa...

Embodiment 3

[0043] 348.5mg ginsenoside Rb 1 , Ginsenoside Rg 1 and notoginsenoside R 1 The mixture (the molar ratio of the three is 44:43:13) and 2.00mmol functional monomer AM were dissolved in 40mL ethanol solvent, dissolved by ultrasonication for 10min, and pre-polymerized for 2-4h. Then add 10.00 mmol of cross-linking agent EGDMA and 74.20 mg of initiator AIBN, and dissolve by ultrasonication for 10 min. The mixed solution was blown with nitrogen for 10 minutes, heated to 60° C. on a constant temperature shaker, and polymerized at 200 rpm for 24 hours.

[0044] Collect by centrifugation and elute with methanol-glacial acetic acid (v / v, 9:1) to remove template molecules until no template molecules can be detected at 204nm by UV spectrophotometer, then wash with methanol until neutral, and then 45°C After vacuum drying for 24 hours, the multi-template monodisperse notoginseng active saponin molecularly imprinted polymer (MIP-3) was obtained.

[0045] No template molecule notoginseng...

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Abstract

The invention discloses a multi-template monodisperse active notoginsenoside molecular imprinted polymer and a preparation method thereof. The method comprises the following steps of mixing and dissolving template molecules and functional monomers into a pore-forming agent, performing uniform ultrasonic mixing to form a template molecule-functional monomer composite, adding an effective amount of initiator and an effective amount of cross-linking agent, and performing ultrasonic nitrogen introduction and sealing; placing a molecular imprinted polymer in a shaking incubator for polymerization reaction under the protection of nitrogen and thermal initiation; after the polymerization reaction is finished, eluting an obtained molecular imprinted polymer by using a methanol-glacial acetic acid to remove the template molecules, washing the molecular imprinted polymer to be neutral by using methanol, and performing vacuum drying to obtain the multi-template monodisperse active notoginsenoside molecular imprinted polymer. Prepared precipitated molecular imprinted polymer microspheres have the advantages of high monodispersity, uniform particle diameter, size controllability, simplicity in preparation, high yield and the like; damage to binding sites and imprinted cavities is reduced, and high affinity and high selectivity of the polymer to the template molecules are ensured.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a preparation method of a molecularly imprinted polymer, in particular to a multi-template monodisperse notoginseng active saponin molecularly imprinted polymer and a preparation method thereof. Background technique [0002] Panax notoginseng (Panax notoginseng (Burk.) F.H.Chen) is the dry root and rhizome of the Araliaceae plant, which has the functions of enhancing body function, increasing coronary blood flow, dilating blood vessels, reducing myocardial oxygen consumption, inhibiting platelet aggregation, reducing Blood viscosity and other effects, is one of the traditional precious medicinal materials in our country. The main active ingredient of notoginseng is notoginseng saponin R 1 , Ginsenoside Rg 1 and ginsenoside Rb 1 , wherein ginsenoside Rg 1 and ginsenoside Rb 1 content is higher. Since ancient times, it has been highly praised by many doctors...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/56C08F220/06C08F226/02C08F226/06C08J9/28C08J9/26B01J20/26B01J20/30
Inventor 陈立娜何宏亮高艳坤
Owner NANJING MEDICAL UNIV
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