Watermelon vine extract with inflammation-diminishing and pain-relieving effects, as well as preparation method and application thereof
An anti-inflammatory, analgesic, and watermelon vine technology, applied in the field of watermelon vine extract and its preparation, can solve problems that have not yet been reported in research
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[0016] One, the preparation of watermelon vine extract
[0017] Such as figure 1 As shown, take 10kg of dried and pulverized watermelon vines, ultrasonicate for 2 hours with an ethanol solution with a volume concentration of 80% according to the weight to volume ratio of 5:1, then soak for 10 days, filter, combine the filtrates, concentrate under reduced pressure to obtain a dark green extract, and freeze Dry to get 300g green powder; disperse the green powder in 5000mL deionized water according to the weight-to-volume ratio of 3:50, let it stand overnight, and centrifuge (3000rpm·min -1 ) to separate the water-insoluble green viscous solid suspension and aqueous solution; take the aqueous solution and use 3 times the volume of petroleum ether extraction, ethyl acetate, and n-butanol to extract the sample successively, and the extract is concentrated under reduced pressure respectively, and the solvent is recovered and evaporated to dryness. Petroleum ether fraction (30 g), e...
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