Endomucin antibody medicament for prompting bone marrow regeneration

An antibody drug, bone marrow regeneration technology, applied in the direction of determination/examination of antibodies, drug combinations, microorganisms, etc., to achieve the effect of promoting bone marrow regeneration function, huge clinical application and research value, and promoting recovery

Active Publication Date: 2014-04-02
ZHONGYI ANKE BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, there is no report on the function of Endomucin in bone marrow hematopoiesis

Method used

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  • Endomucin antibody medicament for prompting bone marrow regeneration
  • Endomucin antibody medicament for prompting bone marrow regeneration
  • Endomucin antibody medicament for prompting bone marrow regeneration

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Experimental program
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Embodiment 1

[0027] 1) Cloning, protein expression and purification of Endomucin gene

[0028] The human Endomucin gene (Genebank number: AF205940) was prepared by gene synthesis, and the full-length human Endomucin gene was respectively cloned into the pGEX-2T vector, so that the target gene was connected behind the glutathione sulfhydryl transferase (GST) gene to The form of GST-Endomucin fusion protein was highly expressed in Escherichia coli BL21(DE3). The expression of human Endomucin protein accounted for 33% of the total bacterial protein. Then, the GST-Endomucin fusion protein was purified using the anti-GST affinity chromatography medium Glutathione-Sepharose, and the GST peptide was cut off by thrombin to obtain human Endomucin protein with a purity of more than 98%.

[0029] 2) The promoting effect of human Endomucin immunized mouse serum on bone marrow hematopoiesis

[0030] Human Endomucin protein was injected intramuscularly into mice, each injection was 10ug, and after 3 i...

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Abstract

The invention provides an Endomucin antibody medicament for prompting bone marrow regeneration. Endomucin has a negative regulation effect on the proliferation and differentiation of hematopoietic cells, namely, the Endomucin has an inhibiting effect on the recovery and regeneration of bone marrow, while an Endomucin antibody can weaken the inhibiting effect and promote the recovery of a bone marrow hematopoietic function. When a medicinal preparation prepared from an Endomucin monoclonal antibody prepared via a myeloma cell fusing technology is adopted for treating a mouse undergoing 5-Fu (5-fluorouracil) chemotherapy, the recovery and regeneration of bone marrow cells can be promoted, the survival rate of the mouse undergoing the chemotherapy can be increased remarkably, and a bone marrow regeneration function is promoted remarkably. The Endomucin has great potential clinical application and research values on the aspect of regulation of the bone marrow hematopoietic function.

Description

technical field [0001] The invention provides an Endomucin antibody drug for promoting bone marrow regeneration, which has the function of promoting recovery and regeneration of damaged bone marrow, and belongs to the technical field of biopharmaceuticals. Background technique [0002] Now, radiation therapy and chemical drug therapy are still the main means of treating tumors and preventing tumor recurrence. One of their main toxicity and side effects is to damage the patient's bone marrow system, resulting in suppression of bone marrow hematopoiesis and immune function, leading to anemia, bleeding, infection, etc. in patients. , even life-threatening phenomenon, which seriously affects the curative effect and often leads to the failure of treatment. Therefore, how to effectively promote the recovery and regeneration of the hematopoietic function of the tumor patient's own bone marrow is of great significance. [0003] The damage caused by radiotherapy and chemotherapy to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P7/06C12Q1/68
Inventor 王群刘景晔高俊芳
Owner ZHONGYI ANKE BIOTECH CO LTD
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