A method for detecting related substances in remifentanil hydrochloride bulk drug
A technology of related substances and detection methods, which is applied in the field of detection of related substances in remifentanil hydrochloride raw materials, and can solve problems such as unclear impurity components and content, inability to clearly display specific content, clinical application impact, etc.
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Embodiment 1
[0059]Take 120801 batches of remifentanil hydrochloride raw material about 0.1g, add methanol 10ml to dissolve, as the test solution, inject 10μl, the chromatographic conditions refer to a standard promulgated by the State Food and Drug Administration in 2009, the chromatographic column is ten An octaalkylsilane bonded silica gel (3um) chromatographic column, 0.1m×4.6mm, with a detection wavelength of 220nm, to obtain a chromatogram.
Embodiment 2
[0061] Take 120802 batches of remifentanil hydrochloride raw material about 0.1g, add methanol 10ml to dissolve, as the test solution, inject 10μl, the chromatographic conditions refer to a standard promulgated by the State Food and Drug Administration in 2009, the chromatographic column is ten An octaalkylsilane bonded silica gel (3um) chromatographic column, 0.1m×4.6mm, with a detection wavelength of 220nm, to obtain a chromatogram.
Embodiment 3
[0063] Take 121101 batches of remifentanil hydrochloride raw material about 0.1g, add methanol 10ml to dissolve, as the test solution, inject 10μl, the chromatographic conditions refer to a standard promulgated by the State Food and Drug Administration in 2009, the chromatographic column is ten An octaalkylsilane bonded silica gel (3um) chromatographic column, 0.1m×4.6mm, with a detection wavelength of 220nm, to obtain a chromatogram.
[0064] From the chromatogram Figure 1-9 , using the detection method of the present invention, each impurity can be separated quickly, and the specific type and content of each impurity can be determined, while referring to the drug standard promulgated by the State Food and Drug Administration in 2009 Figure 10-12 It can be seen that all related substances have not been detected, and the baseline separation is not good, so the detection method of the present invention is of great significance for improving the purity of remifentanil hydrochl...
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