A kind of method for synthesizing pyrroloquinoline quinone by lactic acid bacteria fermentation

Abstract

The invention discloses a method for synthesizing pyrroloquinoline quinone through lactobacillus fermentation. The method comprises the following steps: activating the lactobacillus plantarum PQQ-1 which is stored in an agar subculture medium through an activation culture medium, and then carrying out stationary culture of the lactobacillus plantarum PQQ-1 at a temperature ranging from 20 to 40 DEG C for 16-30h to from primary seeds; further carrying out enlarging stationary culture of the primary seeds at a temperature ranging from 20 to 40 DEG C through a primary seed culture medium for 16-30h to form secondary seeds; and further carrying out enlarging stationary culture of the secondary seeds at a temperature ranging from 20 to 40 DEG C through a secondary seed culture medium for 42-54h and then starting the fermentation stage, and carrying out anaerobic culture in a production fermentation culture medium at a temperature ranging from 20 to 40 DEG C for 54-66h to obtain the PQQ. The used lactobacillus is a food-grade safe microorganism; and the PQQ is obtained through fermentation, and is high in yield and bright in application prospect.

Description

technical field

[0001] The invention relates to the field of microbial fermentation, in particular to a method for synthesizing pyrroloquinoline quinone by fermentation of lactic acid bacteria. Background technique

[0002] Pyrroloquinoline quinone (PQQ for short) is the third prosthetic group found in bacterial dehydrogenase after flavin nucleotide and nicotinamide nucleotide, and its chemical name is 4,5-dihydro -4,5-dioxide-1-hydropyrrole (2,3f)quinone-2,7,9-tricarboxylic acid, also known as Methaxatin. Under natural conditions, only a few bacteria such as Methylovorus, Deinococcus radiodurans, Gluconobacter oxydans, and Klebsiella pneumoniae can synthesize it (Xiong et al, 2011; Magnusson et al, 2007; Khairnar et al, 2003; Felder et al, 2000).

[0003] Although only a few bacteria can synthesize PQQ, trace amounts of PQQ are found in various plants, animals and humans. Japanese scientists determined the PQQ content in 26 common foods and found that the PQQ content in 1...

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