Method for separating and purifying succinic acid from fermentation broth

Abstract

The invention belongs to a technology for separating succinic acid from fermentation products, and specifically relates to a method for separating and purifying succinic acid from fermentation broth. According to the invention, separation and purification of succinic acid can be realized by the operations of appropriate units and effective combinations of the units, such that defects of existing succinic acid separation and purification technologies of complicated process and poor economical efficiency can be overcome. Therefore, a novel economic technology for separating and purifying succinic acid from fermentation broth is established. Specifically, the fermentation broth is clarified through ultrafiltration. Resin decoloration is used for replacing traditional active carbon decoloration, such that the final succinic acid yield can be improved. Succinic acid crystals are directly obtained through crystallization. With the technology, the purity of the separated succinic acid reaches 99.5%, and the yield is higher than 90%.

Description

Technical field

[0001] The present invention relates to a process for producing succinic acid, in particular, the present invention relates to a process for separating and purifying succinic acid from fermentation broth. Background technique

[0002] Succinic Acid, also known as succinic acid, is widely present in organisms. It is an important dibasic organic carboxylic acid and is an important C 4 As a platform compound, succinic acid can participate in many types of reactions and synthesize a variety of chemical products such as 1,4-butanediol (BDO), γ-butyrolactone (GBL), tetrahydrofuran (THF), etc., so it is used in many The field has a wide range of uses, such as as a tranquilizer in the medical field, food additives in the food industry, and surfactants.

[0003] At present, the industrial production of succinic acid is catalyzed by the hydrogenation of maleic anhydride obtained from fossil raw materials. Chemical synthesis methods have increasingly exposed many problems, suc...

Examples

Embodiment 1

[0065] The succinic acid fermentation broth using glucose as the carbon source and Actinobacillus succinate BE-1 as the production strain. The succinic acid concentration is 45 g / L, the formic acid concentration is 8 g / L, the acetic acid concentration is 10 g / L, and the lactic acid The concentration is 14g / L, the amount of residual sugar is small, and the pH of the fermentation broth is 6.8.

[0066] The fermentation broth is subjected to ultrafiltration operation. The ultrafiltration operation adopts hollow fiber membrane equipment. The membrane material is PES membrane. The membrane cut-off separation capacity is 10kDa. The cross-flow operation is adopted. The operating temperature is 25℃, the operating pressure is 0.2MPa, and the After filtration, the protein removal rate was 91%. At this time, the succinic acid concentration was 43g / L. The succinic acid was decolorized by D301 resin. The decolorization temperature was 40°C. After decolorization, the fermentation broth was almo...

Embodiment 2

[0069] The succinic acid fermentation broth with glucose as the carbon source and Actinobacillus succinate BE-1 as the production strain. The succinic acid concentration is 52g / L, the formic acid concentration is 9g / L, the acetic acid concentration is 7g / L, and the lactic acid The concentration is 17g / L, the amount of residual sugar is small, and the pH of the fermentation broth is 6.6.

[0070] The fermentation broth is processed according to two schemes, scheme one: fermentation broth-centrifugation-decolorization-vacuum distillation concentration-crystallization; scheme two: fermentation broth-ultrafiltration-decolorization-vacuum distillation concentration-crystallization;

[0071] Solution 1: Centrifuge the fermentation broth obtained above at a speed of 6000r / min and a centrifugal time of 30min. After centrifugation, collect the supernatant and use D301 resin to decolorize succinic acid. The decolorization temperature is 40℃. After decolorization, the fermentation broth Almos...

Embodiment 3

[0075] The succinic acid fermentation broth using glucose as the carbon source and Actinobacillus succinate-producing BE-1 as the production strain. The concentration of succinic acid is 62g / L, the concentration of formic acid is 10g / L, the concentration of acetic acid is 6g / L, and the lactic acid The concentration is 18g / L, there is no residual sugar, and the pH of the fermentation broth is 6.8.

[0076] Two schemes are used for clarification of fermentation broth, scheme one: adopts microfiltration first and then ultrafiltration; scheme two: fermentation broth is directly clarified by ultrafiltration.

[0077] Option 1: Perform microfiltration-ultrafiltration operations on the fermentation broth at the same time for comparison. The microfiltration operation uses a hollow fiber microfiltration membrane, the pore size of the microfiltration membrane is 0.1μm, the membrane material is PE membrane, and the cross-flow operation is adopted. The operating temperature is 25℃, the membran...

Technical field

[0001] The present invention relates to a process for producing succinic acid, in particular, the present invention relates to a process for separating and purifying succinic acid from fermentation broth. Background technique

[0002] Succinic Acid, also known as succinic acid, is widely present in organisms. It is an important dibasic organic carboxylic acid and is an important C 4 As a platform compound, succinic acid can participate in many types of reactions and synthesize a variety of chemical products such as 1,4-butanediol (BDO), γ-butyrolactone (GBL), tetrahydrofuran (THF), etc., so it is used in many The field has a wide range of uses, such as as a tranquilizer in the medical field, food additives in the food industry, and surfactants.

[0003] At present, the industrial production of succinic acid is catalyzed by the hydrogenation of maleic anhydride obtained from fossil raw materials. Chemical synthesis methods have increasingly exposed many problems, suc...

Examples

Embodiment 1

[0065] The succinic acid fermentation broth using glucose as the carbon source and Actinobacillus succinate BE-1 as the production strain. The succinic acid concentration is 45 g / L, the formic acid concentration is 8 g / L, the acetic acid concentration is 10 g / L, and the lactic acid The concentration is 14g / L, the amount of residual sugar is small, and the pH of the fermentation broth is 6.8.

[0066] The fermentation broth is subjected to ultrafiltration operation. The ultrafiltration operation adopts hollow fiber membrane equipment. The membrane material is PES membrane. The membrane cut-off separation capacity is 10kDa. The cross-flow operation is adopted. The operating temperature is 25℃, the operating pressure is 0.2MPa, and the After filtration, the protein removal rate was 91%. At this time, the succinic acid concentration was 43g / L. The succinic acid was decolorized by D301 resin. The decolorization temperature was 40°C. After decolorization, the fermentation broth was almo...

Embodiment 2

[0069] The succinic acid fermentation broth with glucose as the carbon source and Actinobacillus succinate BE-1 as the production strain. The succinic acid concentration is 52g / L, the formic acid concentration is 9g / L, the acetic acid concentration is 7g / L, and the lactic acid The concentration is 17g / L, the amount of residual sugar is small, and the pH of the fermentation broth is 6.6.

[0070] The fermentation broth is processed according to two schemes, scheme one: fermentation broth-centrifugation-decolorization-vacuum distillation concentration-crystallization; scheme two: fermentation broth-ultrafiltration-decolorization-vacuum distillation concentration-crystallization;

[0071] Solution 1: Centrifuge the fermentation broth obtained above at a speed of 6000r / min and a centrifugal time of 30min. After centrifugation, collect the supernatant and use D301 resin to decolorize succinic acid. The decolorization temperature is 40℃. After decolorization, the fermentation broth Almos...

Embodiment 3

[0075] The succinic acid fermentation broth using glucose as the carbon source and Actinobacillus succinate-producing BE-1 as the production strain. The concentration of succinic acid is 62g / L, the concentration of formic acid is 10g / L, the concentration of acetic acid is 6g / L, and the lactic acid The concentration is 18g / L, there is no residual sugar, and the pH of the fermentation broth is 6.8.

[0076] Two schemes are used for clarification of fermentation broth, scheme one: adopts microfiltration first and then ultrafiltration; scheme two: fermentation broth is directly clarified by ultrafiltration.

[0077] Option 1: Perform microfiltration-ultrafiltration operations on the fermentation broth at the same time for comparison. The microfiltration operation uses a hollow fiber microfiltration membrane, the pore size of the microfiltration membrane is 0.1μm, the membrane material is PE membrane, and the cross-flow operation is adopted. The operating temperature is 25℃, the membran...

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