A kind of water-soluble porphyrin conjugated polymer and its preparation method and application
A conjugated polymer, water-soluble technology, applied in the field of water-soluble porphyrin-conjugated polymers and its preparation, luminescent biosensors, can solve the problem of scarcity of water-soluble porphyrin-conjugated polymers
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Embodiment 1
[0034] Example 1: Preparation of 5,15-di-p-bromophenyl-10,20-di-p-n-hexylphenyl zinc porphyrin monomer (Porphyrinmonomer)
[0035] (1) Preparation of p-bromophenyl dipyrromethane was prepared according to the method disclosed in literature DengLJ, GuZJ, CaoZC, ShenP, LiXL, ZhangLJ, TanST.Mater.Sci.Eng.B2012,177:1641-1648.
[0036] Dissolve 10mmol of p-n-hexylbenzaldehyde and 10mmol of p-bromophenyldipyrromethane in 500mL of dichloromethane, flow argon for 10 minutes, add 10mmol of trifluoroacetic acid, stir at room temperature for 30 minutes, and then add 0.1mmol of p-tetrachlorobenzene quinone, stirred at room temperature for 1 hour, then neutralized by adding 20 mmol triethylamine, removed the solvent by rotary evaporation, then added 60 mL of methanol solution containing 12 mmol zinc acetate, heated to reflux, reacted for 2 hours, and obtained 5,15-di-p-bromo Phenyl-10,20-di-p-n-hexylphenyl zinc porphyrin monomer (23%).
[0037] Such as figure 1 As shown, in DMF solution,...
Embodiment 2
[0038] Embodiment 2: Preparation of water-soluble porphyrin conjugated polymer P1
[0039] (1) Dissolve 0.1mmol 5,15-di-p-bromophenyl-10,20-di-p-n-hexylphenyl zinc porphyrin in 20mL tetrahydrofuran, add 10.0mmol 9,9'-di-n-hexyl-2,7-diboron Acetate fluorene and 9.9mmol 1,4-dibromo-2,5-bis(N,N-diethylamino)alkoxybenzene, argon for 15 minutes, add 0.8mmol tetrakistriphenylphosphorous palladium catalyst and 5mL of 0.1mol / L potassium carbonate aqueous solution, continued to flow argon for 20 minutes, heated and refluxed for 24 hours under the protection of argon, added methanol for precipitation, filtered to obtain the precipitate, and used methanol, n-hexane, and The precipitate was washed with chloroform, the chloroform phase was collected, precipitated with methanol, filtered with suction, and dried to obtain neutral polymer N1 (56%).
[0040] (2) Dissolve 100 mg of N1 in 20 mL of THF, add 0.5 mL of iodomethane, stir at room temperature for 24 hours, filter the precipitate, was...
Embodiment 3
[0042] Embodiment 3: Preparation of water-soluble porphyrin conjugated polymer P2
[0043] (1) Dissolve 0.5mmol 5,15-di-p-bromophenyl-10,20-di-p-hexylphenyl zinc porphyrin in 20mL tetrahydrofuran, add 10.0mmol 9,9'-di-n-hexyl-2,7-diboron Acetate fluorene and 9.5mmol 1,4-dibromo-2,5-bis(N,N-diethylamino)alkoxybenzene, argon flowed for 15 minutes, and 0.8mmol tetrakistriphenylphosphorous palladium catalyst and 5mL of 0.1mol / L potassium carbonate aqueous solution, continued to flow argon for 20 minutes, heated to reflux under the protection of argon for 28 hours, added methanol for precipitation, filtered to obtain the precipitate, and used methanol, n-hexane, and The precipitate was washed with chloroform, the chloroform phase was collected, precipitated with methanol, filtered with suction, and dried to obtain neutral polymer N2 (51%).
[0044] (2) Dissolve 100 mg of N2 in 20 mL of THF, add 0.5 mL of iodomethane, stir at room temperature for 24 hours, filter the precipitate, w...
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