A Strain of High Temperature Cellulose Degrading Bacteria and Its Application
A cellulose-degrading bacteria and cellulose-degrading technology, which is applied in the field of environmental microorganisms, can solve problems such as decreased thermal stability, achieve the effects of low cost, speed up the composting process, and quickly adapt to the high-temperature composting environment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Example 1: Screening and identification of high temperature resistant cellulose degrading bacteria
[0022] Weigh 10g of garden waste compost samples in the high temperature period, place them in a conical flask filled with 10 glass beads and 90ml of sterile water, shake on a shaker at 200rpm for 30min to fully disperse the samples, and let stand at 70°C Enrichment culture for 12h. Use a sterile pipette to draw 1ml of the supernatant and transfer it to the Hutchinson culture medium, and culture at 70°C for about 30 days. Take 10ml of the acclimatization solution and transfer it to a new Hutcheson culture solution, and continue the acclimatization culture, so that it can be subcultured for 3 times.
[0023] The ingredients of the Hutchinson medium above are: Potassium dihydrogen phosphate 1.0g, Sodium chloride 0.1g, Sodium nitrate 2.5g, Magnesium sulfate 0.3g, Ferric chloride 0.01g, Calcium chloride 0.1g, Starch-free filter paper 10g, 1000ml deionized water, pH7.2.
...
Embodiment 2
[0040] Embodiment 2: the preparation of solid bacterial agent
[0041] (1) slant activation: take the slant of the microorganisms of the present invention stored at 4°C and inoculate them into LB solid slant medium, and culture them in a thermostat at 70°C for 18 hours to realize the activation of the slant.
[0042] (2) Preparation of seed solution: Inoculate one slant of the strain activated by the slant in step (1) into 1L of sterile LB liquid culture medium, and shake and cultivate on a water bath shaker at a culture temperature of 70°C and an oscillation frequency of 100rpm. Seed solution was obtained after culturing for 30 h.
[0043] (3) Preparation of fermented seed liquid: the above seed liquid was inoculated into a sterilized fermenter at an inoculum amount of 10% (v / v) for expanded fermentation culture. Under the conditions of a temperature of 70° C. and an oscillation frequency of 120 rpm, the fermented seed liquid was obtained after culturing for 48 hours.
[00...
Embodiment 3
[0045] Embodiment 3: Effect test applied to high temperature composting
[0046] Use garden waste as the main composting material, supplemented with fresh chicken manure, so that the carbon-nitrogen ratio of the mixed material is 25-35:1, and the moisture content is 55-60%. When the maximum temperature of the compost rises to 55°C, the solid bacterial agent prepared in Example 2 is inoculated into the compost material according to the proportion of 15% of the total material, and the carbon-nitrogen ratio is adjusted to 35:1, and the moisture content is 55%. 50d of compost. Simultaneously, the test was carried out without adding the microbial inoculant of the present invention as a control.
[0047] Heap temperature detection: measure once a day for the first 15 days, and measure once every 5 days after that. Turn the pile once every 5 days, turn the pile when the pile temperature reaches 70°C, and keep the pile body temperature between 50-70°C during the high temperature sta...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| length | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More