Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapidly determining content of ractopamine in animal-originated product

A ractopamine, animal-derived technology, used in color/spectral property measurement, measurement devices, instruments, etc., can solve problems such as low sensitivity, high detection limit, and long sample processing time

Inactive Publication Date: 2014-05-28
甘肃省商业科技研究所有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the ELISA indirect competition method is used to determine the content of ractopamine in animal-derived products, there are problems such as long sample processing time, low sensitivity, high detection limit, and high detection cost.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly determining content of ractopamine in animal-originated product
  • Method for rapidly determining content of ractopamine in animal-originated product
  • Method for rapidly determining content of ractopamine in animal-originated product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062]Accurately weigh 4.0000g of sodium chloride, 1.4500g of disodium hydrogen phosphate dodecahydrate, 0.1000g of potassium chloride and 0.1000g of potassium dihydrogen phosphate, mix them with ultrapure water and dissolve them to 500mL to obtain a phosphate buffer Accurately weigh 0.3975g of sodium carbonate and 0.7425g of sodium bicarbonate, dissolve with ultrapure water and settle to 500mL after mixing to obtain carbonate buffer; accurately weigh 2.5514g of citric acid monohydrate and hydrogen phosphate dodecahydrate Disodium 9.1951g, after mixing, dissolve with ultrapure water and set the volume to 500mL to obtain the substrate buffer; accurately weigh 1g of bovine serum albumin, and set the volume to 100mL with phosphate buffer to obtain the BSA blocking solution; in 500mL Add 250 μL Tween-20 to the phosphate buffer to obtain the PBST washing solution; accurately weigh 0.0100 g of 3,3',5,5'-tetramethylbenzidine, dissolve it in 2 mL of dimethyl sulfoxide to obtain TMB Su...

Embodiment 2

[0066] The method of Example 1 was used to detect the content of ractopamine in pure lean meat, except that when preparing the sample preparation solution, ultrasonic extraction was performed for 10 minutes.

Embodiment 3

[0068] The method of Example 1 was used to detect the content of ractopamine in pure lean meat, except that ultrasonic extraction was performed for 8 minutes when preparing the sample preparation solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for rapidly determining the content of ractopamine in an animal-originated product. the method comprises the following steps: preparing nine ractopamine standard solutions from a ractopamine standard stock solution, respectively mixing the nine ractopamine standard solutions with an antibody solution with the equal volume, drying and incubating, adding to an enzyme label plate, drying and incubating, washing the plate, beating dry, adding a secondary antibody solution into the enzyme label plate, washing the plate after the reaction, and beating dry; adding a color developer, drying and incubating, and fully developing; adding a sulfuric acid stopping solution, determining light absorption value of each pore with ELIASA wave, and drawing working curves of the series ractopamine standard solutions; preparing a sample preparation solution from pure lean meat to be determined, determining light absorption value of each pore after processing, and simultaneously carrying out a blank experiment; and calculating the content of ractopamine in the animal-originated product. By the rapid determination method, sensitivity can be raised and energy can be saved. The method has good stability and reproducibility and high recovery rate, and can meet detection requirement of a lab.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and relates to a method for determining the content of β-adrenoreceptor agonists in animal-derived products, in particular to a rapid determination method for the content of ractopamine in animal-derived products. Background technique [0002] Ractopamine is a synthetic β-adrenergic receptor agonist used by some pig farms as a new type of clenbuterol. It can accelerate the growth rate of livestock and poultry, reduce the fat content of carcass, and increase the lean meat rate. Symptoms of poisoning will appear after people eat animal tissues with residual ractopamine, which is more harmful to patients suffering from high blood pressure, glaucoma, diabetes, prostatic hypertrophy and other diseases, and severe cases may be life-threatening. The scope of use of this drug in the aquaculture industry and the regulations of various countries in the world are not the same. Since Decembe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/558G01N21/31
CPCG01N33/9413G01N21/31
Inventor 周鑫櫆洪霞钱滢文
Owner 甘肃省商业科技研究所有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com