Method for rapidly determining content of ractopamine in animal-originated product

A ractopamine, animal-derived technology, used in color/spectral property measurement, measurement devices, instruments, etc., can solve problems such as low sensitivity, high detection limit, and long sample processing time

CN103823056AInactive Publication Date: 2014-05-28甘肃省商业科技研究所有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2014-05-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for rapidly determining the content of ractopamine in an animal-originated product. the method comprises the following steps: preparing nine ractopamine standard solutions from a ractopamine standard stock solution, respectively mixing the nine ractopamine standard solutions with an antibody solution with the equal volume, drying and incubating, adding to an enzyme label plate, drying and incubating, washing the plate, beating dry, adding a secondary antibody solution into the enzyme label plate, washing the plate after the reaction, and beating dry; adding a color developer, drying and incubating, and fully developing; adding a sulfuric acid stopping solution, determining light absorption value of each pore with ELIASA wave, and drawing working curves of the series ractopamine standard solutions; preparing a sample preparation solution from pure lean meat to be determined, determining light absorption value of each pore after processing, and simultaneously carrying out a blank experiment; and calculating the content of ractopamine in the animal-originated product. By the rapid determination method, sensitivity can be raised and energy can be saved. The method has good stability and reproducibility and high recovery rate, and can meet detection requirement of a lab.
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Description

technical field

[0001] The invention belongs to the technical field of food safety detection, and relates to a method for determining the content of β-adrenoreceptor agonists in animal-derived products, in particular to a rapid determination method for the content of ractopamine in animal-derived products. Background technique

[0002] Ractopamine is a synthetic β-adrenergic receptor agonist used by some pig farms as a new type of clenbuterol. It can accelerate the growth rate of livestock and poultry, reduce the fat content of carcass, and increase the lean meat rate. Symptoms of poisoning will appear after people eat animal tissues with residual ractopamine, which is more harmful to patients suffering from high blood pressure, glaucoma, diabetes, prostatic hypertrophy and other diseases, and severe cases may be life-threatening. The scope of use of this drug in the aquaculture industry and the regulations of various countries in the world are not the same. Since Decembe...

Examples

Embodiment 1

[0062]Accurately weigh 4.0000g of sodium chloride, 1.4500g of disodium hydrogen phosphate dodecahydrate, 0.1000g of potassium chloride and 0.1000g of potassium dihydrogen phosphate, mix them with ultrapure water and dissolve them to 500mL to obtain a phosphate buffer Accurately weigh 0.3975g of sodium carbonate and 0.7425g of sodium bicarbonate, dissolve with ultrapure water and settle to 500mL after mixing to obtain carbonate buffer; accurately weigh 2.5514g of citric acid monohydrate and hydrogen phosphate dodecahydrate Disodium 9.1951g, after mixing, dissolve with ultrapure water and set the volume to 500mL to obtain the substrate buffer; accurately weigh 1g of bovine serum albumin, and set the volume to 100mL with phosphate buffer to obtain the BSA blocking solution; in 500mL Add 250 μL Tween-20 to the phosphate buffer to obtain the PBST washing solution; accurately weigh 0.0100 g of 3,3',5,5'-tetramethylbenzidine, dissolve it in 2 mL of dimethyl sulfoxide to obtain TMB Su...

Embodiment 2

[0066] The method of Example 1 was used to detect the content of ractopamine in pure lean meat, except that when preparing the sample preparation solution, ultrasonic extraction was performed for 10 minutes.

Embodiment 3

[0068] The method of Example 1 was used to detect the content of ractopamine in pure lean meat, except that ultrasonic extraction was performed for 8 minutes when preparing the sample preparation solution.