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Detection tube for detecting content of hydrogen in water and application of detection tube

A detection tube and sample detection technology, applied in the field of detection tubes, can solve the problems of cumbersome and harsh operations, no rapid detection kits, and inability to apply real-time rapid detection, etc.

Active Publication Date: 2014-06-04
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to seek a fast and sensitive qualitative and semi-quantitative method to solve the problem that the current detection methods of the concentration of hydrogen molecules in sample water need certain instruments and equipment and the operation is cumbersome and harsh, which cannot be applied to real-time rapid detection, and there is currently no rapid Problems with kits for detecting hydrogen concentration in samples

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  • Detection tube for detecting content of hydrogen in water and application of detection tube
  • Detection tube for detecting content of hydrogen in water and application of detection tube

Examples

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Embodiment 1

[0022] The inner diameter of the centrifuge tube is 0.5cm, the length is 3cm, and there is a scale line at 2.55cm. Accurately weigh 0.029mg of thioacetylcholine and 0.024mg of DTNB, of which 0.1μg of acetylcholinesterase (from electric eel, with a total activity of 827U / mg) , add an appropriate amount of disodium hydrogen phosphate (to make the final solution neutral), put it into a centrifuge tube, mix well, dry, seal and freeze. Use the hydrogen electrode to measure the concentration of the hydrogen water used is 3000μmol / L and 200μmol / L. Use these two different concentrations of hydrogen water and pure water (as a reference) as solvents, respectively take 200μL and add them to the centrifuge tube at the same time, mix well , detected at the visible light absorption value of 412nm.

Embodiment 2

[0024] Accurately weigh 0.029mg of thioacetylcholine and 0.024mg of DTNB, including 0.11μg of acetylcholinesterase (from electric eel, with a total activity of 827U / mg), add an appropriate amount of disodium hydrogen phosphate (so that the pH of the final solution is 6.5) and put them into a centrifuge tube After drying, seal and freeze. Use a hydrogen electrode to measure the concentration of hydrogen water used is 100 μmol / L, use pure water and hydrogen water of this concentration as solvents, respectively take 200 μL and add them to centrifuge tubes at the same time, mix well, and detect at the visible light absorption value of 412nm.

Embodiment 3

[0026] Accurately weigh 0.87mg of thioacetylcholine and 0.24mg of DTNB, including 0.36μg of acetylcholinesterase (from electric eel, with a total activity of 827U / mg), add disodium hydrogen phosphate (to make the final solution pH 7.5) and put it into a centrifuge tube , sealed and frozen. Use a hydrogen electrode to measure the concentration of hydrogen water used is 500 μmol / L, use pure water and hydrogen water of this concentration as solvents, respectively take 200 μL and add them to centrifuge tubes at the same time, mix well, and detect at the visible light absorption value of 412nm.

[0027] Detection steps of the present invention are:

[0028] Or according to the method of the present invention, accurately weigh 0.03mg thioacetylcholine and 0.024mgDTNB, acetylcholinesterase and sodium dihydrogen phosphate respectively, put them into a centrifuge tube, take the same dose of each material in another centrifuge tube, and add them simultaneously Deionized water and sampl...

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Abstract

The invention discloses a detection tube for detecting the content of hydrogen in water and an application of the detection tube and belongs to the field of enzymatic analysis in the technical field of biology. A tube body of the detection tube is a transparent cylinder; one end of the tube body is open and is provided with a sealing cover; acetylcholin esterase, a substrate, a color developing agent and powder of buffer substances are arranged at the bottom of the transparent detection tube; a mass ratio of the acetylcholin esterase to acetylthiocholine is 1:(200-300); a ratio of the acetylthiocholine to the color developing agent DTNB is (1:1)-(1:5); according to the buffer substances, the pH value of the liquid system is 6-8. The application method comprises the following steps: taking hydrogen water and deionized water of different concentrations as solvents, taking the deionized water, respectively adding hydrogen water with different concentrations, as solvents, and deionized water as reference, into centrifuge tubes, standing for 1 minute, observing the color of the reaction solution, and contrasting, wherein the color of the reaction solution is more luminous yellow than a solution treated by the deionized water, and the hydrogen water exists in the sample water; or, performing detection at the visible light absorption value of 412nm, and due to comparison of the deionized water, the higher the absorption value is, the higher the content of the hydrogen in the hydrogen water is.

Description

technical field [0001] The invention discloses a detection tube for detecting hydrogen content in water, which can realize qualitative and rapid identification of hydrogen-containing water and ordinary water, and belongs to the field of enzymatic analysis in the field of biotechnology. Background technique [0002] Hydrogen is a colorless, tasteless, odorless, flammable gas and is the lightest diatomic molecule in nature. In the field of diving medicine, people use hydrogen-oxygen mixture as a breathing medium to shorten the decompression time. For a long time, it has been believed that hydrogen gas is just a metabolic waste gas of intestinal flora and has no biological effect. Therefore, hydrogen has been considered as a physiologically inert gas. In 2007, Japanese scholars published an article in "Natural Science" and found that in vivo models proved that breathing 2% hydrogen can effectively reduce the infarct size caused by cerebral ischemia-reperfusion, and then hydro...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 马雪梅刘梦昱王婷婷
Owner BEIJING UNIV OF TECH
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