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A biological test method for rapid detection of comprehensive toxicity of traditional Chinese medicine

A test method and technology of traditional Chinese medicine, applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems that the consistency and safety of traditional Chinese medicine cannot be effectively guaranteed, and overcome the small number of samples And the effects of individual differences, responsiveness, and large bacterial sample size

Active Publication Date: 2017-04-12
SICHUAN ACAD OF CHINESE MEDICINE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these quality control standards and testing methods are still unable to effectively guarantee the consistency and safety of traditional Chinese medicine efficacy

Method used

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  • A biological test method for rapid detection of comprehensive toxicity of traditional Chinese medicine
  • A biological test method for rapid detection of comprehensive toxicity of traditional Chinese medicine
  • A biological test method for rapid detection of comprehensive toxicity of traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 toxicity detection method of the present invention

[0034] 1 Experimental materials

[0035] 1.1 Strains

[0036] Freeze-dried powder of Vibrio fischeri (CS234) was purchased from Beijing Hamamatsu Photon Technology Co., Ltd., and stored at -20°C in the dark.

[0037] 1.2 Main reagents

[0038] Recovery diluent: 0.51mol / L sodium chloride solution;

[0039] Osmotic adjustment solution: 3.42mol / L sodium chloride solution;

[0040] 1.3 Main instruments and equipment

[0041] LUMIStox300 biological toxicity tester and supporting LUMIStherm preheating tank and test tube (Dr.BrunoLange GmbH), BCD-539WF refrigerator (Haier), PB-10 acidity meter (Sartorius)

[0042] 2 Experimental methods

[0043] 2.1 Preparation of bacteria solution for test

[0044] Take out 1 bottle of Vibrio fischeri freeze-dried powder from the freezer (-20°C) and place it at room temperature (about 20°C) for 15 minutes to equilibrate, then add 0.2-1.0ml recovery diluent and place it a...

Embodiment 2

[0096] Embodiment 2 applies the present invention to carry out comprehensive toxicity test to 10 kinds of Chinese medicines

[0097] 1. Experimental materials

[0098] With embodiment 1.

[0099] Test traditional Chinese medicines: raw tripterygium wilfordii, raw cocklebur, evodia, cnidium, vinegar kansui, vinegar pokeweed, Guanzhong, strychnium chinensis, araceae, and Chonglou, and comprehensive toxicity tests were carried out with water extracts.

[0100] 2. Experimental method

[0101] Adopt the method of embodiment 1, carry out luminous intensity measurement to 10 kinds of Chinese medicines, calculate inhibition rate, use IC 50 The comprehensive toxicity of each sample was compared.

[0102] 2.1 Preparation of bacteria solution for test

[0103] With embodiment 1.

[0104] 2.2 Pre-test

[0105] With embodiment 1.

[0106] 2.3 Preparation of sample solution to be tested

[0107] With embodiment 1.

[0108] 2.4 Preparation of blank control solution

[0109] With em...

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Abstract

The invention discloses a biological testing method for rapidly detecting the comprehensive toxicity of traditional Chinese medicines, which comprises the following steps: (1) preparing the bacterial liquid for testing: taking the freeze-dried powder of luminescent bacteria and resuscitating it with a solution with a chloride ion concentration of 0.1-1.0 mol / L , to obtain the test bacterial solution; (2) Detection: Take the sample of the traditional Chinese medicine to be tested, and use the test bacterial solution to detect, and determine the sample concentration with a luminous intensity inhibition rate of 50% and the concentration-effect kinetic curve. The detection accuracy and sensitivity of the method of the invention are high, and the operation is simple.

Description

technical field [0001] The invention relates to a biological testing method for rapidly detecting the comprehensive toxicity of traditional Chinese medicines. Background technique [0002] Traditional Chinese medicine is a treasure of our country, and it has played a huge role in disease prevention, treatment and health care. With the expansion of the application range of traditional Chinese medicine and its preparations, clinical reports of adverse reactions caused by it are also increasing, and there are reports of fatalities. The incidence of adverse reactions of traditional Chinese medicine is second only to anti-infective drugs in chemical medicines, and there is an upward trend in recent years. . Guan Mu Tong, Guang Fang Ji, Qing Mu Xiang and other aristolochic acid drugs caused severe kidney damage, the tonic Chinese medicine Polygonum multiflorum caused severe liver damage, and the introduction of Chinese medicine injections such as Shuanghuanglian and Qingkailing i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 赵军宁鄢良春
Owner SICHUAN ACAD OF CHINESE MEDICINE SCI