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Medicinal application of chicoric acid

A technology of cichoric acid and medicine, which is applied in the field of cichoric acid as histone deacetylase inhibition, and can solve the problem of not being able to promote virus resistance

Inactive Publication Date: 2014-06-25
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But mixing echinacea extract with mammalian cells does not promote viral resistance

Method used

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  • Medicinal application of chicoric acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The inhibitory effect of cichoric acid on histone deacetylase (HDCA) was observed on H4IIE cells using cell lysates:

[0026] Inoculate H4IIE cells in a 6-well plate as usual. After the cells adhere to the wall, continue to culture until 90% of the area of ​​the well is covered with cells. Add different concentrations (5, 10, 20, 100 μM) of cichoric acid. 4h and 24h, prepare the whole cell lysate, use the bicinchoninic acid method to determine its protein content, take the cell lysate without adding cichoric acid as the blank, and use the cell lysate with sodium butyrate as the positive control, according to Merck Millipore The method described in the instruction manual of the Histone Deacetylase Assay Kit (Histone Deacetylase Assay Kit (HDAC), catalog number: 17-320) produced by the company was used to determine the activity of histone deacetylase, and the results are as follows:

[0027] The inhibitory effect of cichoric acid on HDAC was determined by H4IIE cell lysat...

Embodiment 2

[0032] The inhibitory effect of cichoric acid on histone deacetylase (HDCA) was observed in H4IIE cells using nuclein:

[0033] Inoculate H4IIE cells in a 6-well plate as usual. After the cells adhere to the wall, continue to culture until 90% of the area of ​​the well is covered with cells. , 24h, extract the nuclear protein of the cells, use the bicinchoninic acid method to measure its protein content, take the nuclear protein without adding cichoric acid as a blank, and take the nuclear protein with sodium butyrate as a positive control, according to the production of Merck Millipore The method described in the instructions of the Histone Deacetylase Assay Kit (Histone Deacetylase Assay Kit (HDAC), catalog number: 17-320) was used to determine the activity of histone deacetylase, and the results were as follows:

[0034] The inhibitory effect of cichoric acid on HDAC was determined by H4IIE nucleoprotein (cells were treated for 2h)

[0035] medicine

Inhibition...

Embodiment 3

[0041] The inhibitory effect of cichoric acid on histone deacetylase (HDCA) was observed on L6 cells using cell lysates:

[0042] Inoculate L6 cells in a 6-well plate as usual. After the cells adhere to the wall, continue to culture until 90% of the area of ​​the well is covered with cells. , 24h, prepare the whole cell lysate, use the bicinchoninic acid method to measure its protein content, take the cell lysate without adding cichoric acid as the blank, and take the cell lysate with sodium butyrate as the positive control, according to Merck Millipore The method described in the instruction manual of the production of Histone Deacetylase Assay Kit (Histone Deacetylase Assay Kit (HDAC), catalog number: 17-320) was used to measure the activity of histone deacetylase, and the results are as follows:

[0043] The inhibitory effect of cichoric acid on HDAC was determined by L6 cell lysate (cells were treated for 4h)

[0044] medicine

Inhibition rate(%)

Posit...

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Abstract

The invention relates to a medicinal application of chicoric acid and application of anti-tumor medicaments and health products formed by chicoric acid. In-vitro tests prove that chicoric acid has the effect of a histone deacetylase (HDAC) inhibitor.

Description

technical field [0001] The present invention relates to the use of cichoric acid as an inhibitor of histone deacetylases. It is confirmed by in vitro experiments that cichoric acid has the inhibitory effect on histone deacetylase, and can be used as an antitumor drug and a health care product. Background technique: [0002] Chichoric acid filamentous, needle-shaped crystals. Molecular formula C 22 h 18 o 12 , with a melting point of 206°C and an optical rotation of [α]+383.5° (MeOH, C: 1.555mol / l). Density 1.641g / cm 3 . Soluble in ethanol, methanol, dioxane and acetone, slightly soluble in ethyl acetate and ether, insoluble in light petroleum, benzene and chloroform. At the same time, cichoric acid is easily soluble in hot water, but only 0.5% is soluble at a temperature of 20 degrees Celsius. Its sodium salt is soluble in water, barium salt is slightly soluble in water, and yellow lead salt is insoluble in water. When cichoric acid and ferric chloride are mixed, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A23L1/29A61P35/00A23L33/00
Inventor 信学雷阿吉艾克拜尔·艾萨蒋岚叶建平
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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