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Compound biological green manure optimized culturing method

A green manure and biological technology, applied in the field of optimized cultivation of compound biological green manure, can solve problems such as not easy to be harmless, inconvenient to use, and policies to reduce the use of agrochemicals, and achieve good ecological restoration function, comprehensive nutritional components, rapid and efficient growth reproductive effect

Inactive Publication Date: 2014-07-16
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the above-mentioned deficiencies existing in the prior art, and proposes a method for optimizing the cultivation of compound biological green manure, aiming at the concept of green food production process that has attracted public attention at present, the policy of reducing the use of agricultural chemicals, and the season of duckweed Sexual growth and reproduction, difficult to harmless, inconvenient to use, etc., the invention can make duckweed reproduce quickly and efficiently, and efficiently convert nutrients in chemicals into safer and more effective duckweed green manure after application

Method used

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  • Compound biological green manure optimized culturing method
  • Compound biological green manure optimized culturing method
  • Compound biological green manure optimized culturing method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The main materials of this embodiment: sodium hydroxide, hydrochloric acid, complete culture solution, green duckweed, few-rooted purple duckweed, and many-rooted purple duckweed.

[0031] Such as figure 1 As shown, the present embodiment comprises the following steps: the pH of the complete culture solution is adjusted to 4, 5, 6, 7, 8, 9, 10, 11 (wherein 6 to 11 are used as comparative examples) with sodium hydroxide and hydrochloric acid solution , transfer well-growing Qingping, less-rooted and multi-rooted Zipingia (the fresh weight ratio of Qingping: less-rooted Ziding: multi-rooted Ziding is about 9:3:1) into the culture medium containing 200mL Disposable transparent plastic cups with a capacity of 350 mL were placed in a culture room with a temperature of 30 °C and a light intensity of 2790 Lx for 7 days.

[0032] Such as Figure 4As shown, the growth measurement of duckweed: 1). The measurement of frond width: the ruler directly measures the maximum width of ...

Embodiment 2

[0036] The main materials of this embodiment: complete culture solution, Qingping, few-rooted Ziping, and many-rooted Ziping.

[0037] In this embodiment, the well-growing Qingping, less-rooted and multi-rooted Ziping (the fresh weight ratio of Qingping: less-rooted and multi-rooted Ziding is about 9:3:1) was transferred into a 200mL culture medium. The capacity of the culture medium is 350mL in a disposable transparent plastic cup, the pH of the culture medium is 4.5, and then put into an incubator with a light intensity of 3000Lx, and the temperature is set to 10°C, 15°C, 20°C, 25°C, 30°C , 35°C, and 40°C for 7 days. Other culture conditions are: the light intensity is 2790lux, and the light-dark ratio is 16h:12h.

[0038] The growth assay of duckweed and the activity assay of the enzymatic defense system are the same as in Example 1.

[0039] In this example, when the temperature was 30°C, the growth of multi-rooted duckweed, few-rooted duckweed and Qingping appeared peak...

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Abstract

Provided is a compound biological green manure optimized culturing method. Adversity simulation processing is carried out on lemna minor matched according to different varieties, and then adequate nutrients and proper light are provided to achieve the aim of efficient and quick culturing. The lemna minor comprises duckweed, spirodela oligorrhiza and multi-root lemna minor. According to the concept, which the public pay much attention to, about the production process of green food, agrochemical use policies and the problems of seasonal growth and breeding of the lemna minor, harmless performance difficult to achieve and use inconvenience, by means of the method, quick and efficient reproduction of the lemna minor can be achieved, and nutrient substance in chemicals can be used for culturing of lemna minor green manure more safely and efficiently after being efficiently converted and applied.

Description

technical field [0001] The invention relates to a method in the technical field of plant planting, in particular to an optimized planting method of compound biological green manure. Background technique [0002] Duckweed green manure varieties are a common class of small floating aquatic angiosperms described in "Flora of China", and have been described in "Lei Huang, YanyanLua and Xu Gaoa etc.; Ammonium‐induced oxidative stress on plant growth and antioxidant response of duckweed (Lemna minor L.), Ecological Engineering, 2013, (58):355‐362, Risky Ayu Kristanfi, Masahiro Kanbe, and Tadashi Toyama; Accelerated biodegradation of nitrogenols in the rhizosphere of Spirodelapolyrrhiza, Journal of Environmental Sci12 24(5): 800‐807", "Jiele Xu and Genxiang Shen; Effects of Harvest Regime and Water Depth‐on Nutrient Recovery from Swine Wastewater by Growing Spirodelaoligorrhiza, Water Environment Research, 2011, November", "Zhu Yerong, Wang Yong, etc.; Important progress in severa...

Claims

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Application Information

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IPC IPC(8): A01G31/00C05F11/00
Inventor 沈健英黄荣松陈侠桦郭利利
Owner SHANGHAI JIAO TONG UNIV
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