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Organic subculture preservation method for dioscorea bulbifera embryogenic callus

An embryogenic callus and organic technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of short embryogenic callus retention time, supply, and difficult to transgene research

Inactive Publication Date: 2014-07-16
SHANGRAO NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain problems in the subculture preservation of embryogenic callus, such as the short embryogenic retention time of embryogenic callus, and the use of plant hormones causes some embryogenic callus to turn into non-embryogenic callus, Long-term hormonal subculture loss of embryogenic callus regeneration ability, etc., it is difficult to provide enough recipients for transgenic research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0004] The present invention will be further described in conjunction with following examples:

[0005] (1) The first subculture preservation of the embryogenic callus of Huangdu

[0006] In the ultra-clean workbench, the embryogenic callus of Huangdu microtuber was first inserted into medium one (MS+10-20g / L banana juice+5-10g / L potato juice+1-2g / L hydrolyzed casein +0.5-1g / L peptone+30g / L sucrose+7g / L agar, no pH adjustment), after inoculation, put the material into the culture room for culture, the culture conditions of the culture room are: temperature 25±2℃, light time 16h / d , light intensity 1500-25001x;

[0007] (2) The second subculture preservation of the embryogenic callus of Huangdu

[0008] After being cultured on medium one for 60 days, the embryogenic callus of Huang alone was transferred to medium two (MS+10-20g / L banana juice+5-10g / L carrot juice+1-2g / L hydrolyzed milk protein+ 0.5-1g / L peptone + 30g / L sucrose + 7g / L agar, no pH adjustment), after inoculatio...

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Abstract

The invention relates to an organic succeeding preservation method for dioscorea bulbifera embryogenic callus. The organic subculture preservation method comprises the following step: carrying out subculture on the dioscorea bulbifera embryogenic callus by using six subculture preservation culture media containing different organic additives in sequence, wherein the preservation can be kept for five years, and five years later, the dioscorea bulbifera embryogenic callus also can be propagated greatly. Compared with the prior art, the method has the advantages that the regeneration capability of the dioscorea bulbifera embryogenic callus subjected to subculture preservation cannot be reduced, and embryonic performance cannot be lost.

Description

Technical field: [0001] The invention relates to a method for preserving plant callus, in particular to a method for organic subculture preservation of the embryogenic callus of Huangdu. Background technique: [0002] Dioscorea bulbifera L. is a plant of the genus Dioscorea in the family Dioscorea, and its tubers are generally used for medicine, mainly for the treatment of various thyroid diseases and various cancers. The induction of embryogenic callus of Huangdu can provide recipients for the selection of Huangdu mutant strains and transgenic research. Establishing a receptor system with stable genetics, high transformation efficiency and easy regeneration is one of the key technologies in transgenic research. So far, the most effective way to establish a transgenic receptor system is to induce and culture embryogenic callus. However, there are certain problems in the subculture preservation of embryogenic callus, such as the short embryogenic retention time of embryogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 洪森荣尹明华
Owner SHANGRAO NORMAL UNIV
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