Preparation method of hydrophobia inactivated vaccine

Abstract

The invention discloses a preparation method of a hydrophobia inactivated vaccine. The preparation method comprises the following steps of carrying out cell recovery, generation and culture on a hamster kidney cell system, carrying out virus seed reproduction and venom reproduction on the treated hamster kidney cells, preparing a vaccine adjuvant, adding B-propiolactone according to a proportion of 0.025% of the total amount of the venom, inactivating at 4 DEG C for 24h, hydrolyzing at 36 DEG C for 2h and quantificationally sub-packaging the hydrolyzed cell venom and vaccine adjuvant according to a proportion of 9: 1 to obtain the hydrophobia inactivated vaccine. The preparation method of the hydrophobia inactivated vaccine has the advantages that the operation is simple, the process is stable, the virus content of the vaccine is high and the quality is easy to control, and is capable of remarkably improving the yield and quality of the vaccine; the vaccine prepared by the method is good in safety, high in immunity and low in cost.

Description

technical field [0001] The invention relates to a preparation method of an inactivated rabies vaccine. Background technique [0002] At present, the production of inactivated rabies vaccines for dogs in my country is mainly produced by traditional spinner bottle culture cells. This method has high cost, low poison price and high labor intensity. At the same time, because each spinner bottle is an independent unit, the cell quality and virus titer of each bottle are not the same, resulting in unstable quality of the produced vaccines and large differences in batches. Therefore, prior art still needs further improvement and development. Contents of the invention [0003] In view of the above deficiencies in the prior art, the object of the present invention is to provide a preparation method of inactivated rabies vaccine, so as to improve the stability and virus content of the preparation process. [0004] Technical scheme of the present invention is as follows: [0005] ...

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Problems solved by technology

[0002] At present, the production of inactivated rabies vaccines for dogs in my country is mainly produced by traditional spinner bottle culture cells. This method has high cost, low toxicity and high labor intensity.

At the same time, because each spinner bottle is an independent unit, the cell quality and virus titer of each bottle are not the same, resulting in unstable quality of the vaccine produced and large differences in batches

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