Preparation method of hydrophobia inactivated vaccine

An inactivated vaccine and rabies technology, applied in the field of preparation of inactivated rabies vaccine, can solve the problems of unstable vaccine quality, high labor intensity, large batch differences, etc., achieve uniform and stable virus liquid, improve output and quality, and improve manufacturing costs. low effect

Inactive Publication Date: 2014-07-30
JILIN HEYUAN BIOENG LIMITED
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  • Abstract
  • Description
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Problems solved by technology

[0002] At present, the production of inactivated rabies vaccines for dogs in my country is mainly produced by traditional spinner bottle culture cells. This method has high cost, low toxicity and high labor intensity.
At the same time, because each spinner bottle is an independent unit, the cell quality and virus titer of each bottle are not the same, resulting in unstable quality of the vaccine produced and large differences in batches

Method used

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  • Preparation method of hydrophobia inactivated vaccine
  • Preparation method of hydrophobia inactivated vaccine

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Embodiment Construction

[0017] The present invention provides a method for preparing an inactivated rabies vaccine. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0018] The invention provides a kind of preparation method of rabies inactivated vaccine, it comprises the following steps:

[0019] Step 101: performing the steps of recovering, subculture and culturing the hamster kidney cell line;

[0020] Step 102: Then in step: 101, the hamster kidney cells processed carry out the steps of breeding of poisonous species and breeding of venom;

[0021] Step 103: Prepare the vaccine adjuvant, then add B-propiolactone according to the ratio of 0.025% of the total venom, inactivate at 4°C for 24 hours, then hydrolyze at 37°C for 2 hou...

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Abstract

The invention discloses a preparation method of a hydrophobia inactivated vaccine. The preparation method comprises the following steps of carrying out cell recovery, generation and culture on a hamster kidney cell system, carrying out virus seed reproduction and venom reproduction on the treated hamster kidney cells, preparing a vaccine adjuvant, adding B-propiolactone according to a proportion of 0.025% of the total amount of the venom, inactivating at 4 DEG C for 24h, hydrolyzing at 36 DEG C for 2h and quantificationally sub-packaging the hydrolyzed cell venom and vaccine adjuvant according to a proportion of 9: 1 to obtain the hydrophobia inactivated vaccine. The preparation method of the hydrophobia inactivated vaccine has the advantages that the operation is simple, the process is stable, the virus content of the vaccine is high and the quality is easy to control, and is capable of remarkably improving the yield and quality of the vaccine; the vaccine prepared by the method is good in safety, high in immunity and low in cost.

Description

technical field [0001] The invention relates to a preparation method of an inactivated rabies vaccine. Background technique [0002] At present, the production of inactivated rabies vaccines for dogs in my country is mainly produced by traditional spinner bottle culture cells. This method has high cost, low poison price and high labor intensity. At the same time, because each spinner bottle is an independent unit, the cell quality and virus titer of each bottle are not the same, resulting in unstable quality of the produced vaccines and large differences in batches. Therefore, prior art still needs further improvement and development. Contents of the invention [0003] In view of the above deficiencies in the prior art, the object of the present invention is to provide a preparation method of inactivated rabies vaccine, so as to improve the stability and virus content of the preparation process. [0004] Technical scheme of the present invention is as follows: [0005] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/205C12N7/00C12N5/071A61P31/04C12R1/93C12R1/91
Inventor 吴金张中洋高玉龙任培森李晓莉杨金梅孙岩
Owner JILIN HEYUAN BIOENG LIMITED
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