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A Rapid Screening Method for Antibacterially Active Bacteria and Actinomycetes

An antibacterial activity, actinomycetes technology, applied in the field of rapid separation of microorganisms, can solve the problems of high cost, long cycle, high labor intensity, etc., and achieve the effect of increasing probability, simple operation and low labor intensity

Active Publication Date: 2017-06-16
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has high cost, long period and high labor intensity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, a kind of method for fast screening antibacterial activity bacterium and actinomycetes, its steps are as follows:

[0016] (1) Sample collection: collect seawater, sea mud, soil and river water samples with sterile sampling bottles, send them to the laboratory quickly and isolate bacteria and actinomycetes as soon as possible;

[0017] (2) Medium preparation: 2216E medium: peptone 5g, yeast extract 1g, FePO 4 0.1g, Chen Haihai 1000ml; Gaoshi No. 1 medium: soluble starch 20.0g, KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g. NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, distilled water 1000ml, pH7.2; nutrient agar medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000ml, pH7.2; PDA medium: potato 200g, glucose 20g, distilled water 1000ml, natural pH ; If the above medium is a solid medium, add agar 20g / L; except for the 2216E medium, if it is used for the isolation of marine sample strains, the distilled water in the medium is replaced by seawate...

Embodiment 2

[0023] Example 2, step (5) of a method for rapidly screening antibacterially active bacteria and actinomycetes described in Example 1, by intercepting the tip Escherichia coli Co-cultivate with the bacteria or actinomycetes to be screened, and put them on the indicator bacteria plate. After the indicator bacteria are cultivated, measure the diameter of the inhibition zone, and quickly screen the antibacterial active strains according to the presence and size of the inhibition zone.

example example 3

[0024] Example 3, comparative experiment:

[0025] 1. Screening of prior art methods.

[0026] Seawater and sea mud were collected from Liandao, Lianyungang sea area, and soil and freshwater samples were collected from Huaguo Mountain. Bacteria and actinomycetes in marine samples were separated by dilution coating method using 2216E medium and Gaoshi No. 1 medium prepared from seawater. Bacteria and actinomycetes were isolated from land samples using nutrient agar medium and Gao's No. 1 medium. The purified single colonies were inoculated to slant cultures and stored at 4°C. A total of 691 strains of bacteria and 93 strains of actinomycetes were isolated. Different marine bacteria and terrestrial bacteria were inoculated into 2216E medium and nutrient agar medium, and marine actinomycetes and terrestrial actinomycetes were inoculated into Gao’s No. 1 medium prepared by seawater and distilled water, and cultured to a cell volume of 2%. Take 0.2 mL and inoculate 25 mL of fer...

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PUM

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Abstract

The invention relates to a method for rapidly screening antibacterial active bacteria and actinomycetes, the steps of which include (1) sample collection; (2) medium preparation; (3) separation of bacteria and actinomycetes; (4) intercepting tip (5) Screening of strains with antibacterial activity. The method of the present invention rapidly screens antibacterial active bacteria and actinomycetes by means of co-cultivation, compared with purebred culture screening antibacterial active bacteria and actinomycetes, it can increase the probability of novel antibacterial active bacterial strains and antibacterial active substances, and the whole The screening process is fast, the operation is simple, the labor intensity is small, and the bacteria and actinomycetes with antibacterial activity can be screened quickly, intuitively and efficiently, which provides great convenience for the screening research of bacteria and actinomycetes with antibacterial activity.

Description

technical field [0001] The invention relates to a rapid separation method of microorganisms, more specifically a method for rapid screening of antibacterial active bacteria and actinomycetes. Background technique [0002] The long-term and extensive use of antibiotics has led to an increase in the types of drug-resistant pathogenic bacteria, and even the emergence of "super bacteria", and the emergence of new infectious diseases, making the research and development of new antibacterial substances imminent (Li Yuezhong, Chen Qi. Marine Microbial Resources and Studies on the production of bioactive metabolites, Advances in Bioengineering, 2000, 20 (5): 28-31). During the long-term research and development of microbial resources, it is found that it is more and more difficult to discover new antibacterial substances from microbial secondary metabolites. Antibiotic screening methods directly affect the screening efficiency of new antibiotics. (Wang Xing, Wu Wenhui, Chen Zhihua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/19C12R1/445C12R1/44C12R1/80
Inventor 刘姝房耀维王淑军吕明生焦豫良
Owner HUAIHAI INST OF TECH
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