Device and method for measuring diffusion coefficient of dissolved oxygen in biological membrane

A diffusion coefficient and biofilm technology, applied in the field of electrochemical analysis, can solve the problems of inaccurate results, long time-consuming, in-situ measurement of samples, etc., and achieve accurate measurement results, short time-consuming, and small system errors.

Active Publication Date: 2014-08-06
嘉兴华吉环保科技有限公司
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Problems solved by technology

The traditional method of measuring the diffusion coefficient of solid internal substances takes a lon...

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  • Device and method for measuring diffusion coefficient of dissolved oxygen in biological membrane
  • Device and method for measuring diffusion coefficient of dissolved oxygen in biological membrane
  • Device and method for measuring diffusion coefficient of dissolved oxygen in biological membrane

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Embodiment Construction

[0036] The present invention will be described in further detail below in conjunction with specific examples.

[0037] like figure 1 As shown, a device for measuring the diffusion coefficient of dissolved oxygen in a biofilm includes a gas delivery device, an inlet humidification bottle 5, a biofilm test chamber 6 and a dissolved oxygen microelectrode system;

[0038] Described biofilm test chamber 6 is a cuboid transparent plexiglass chamber, is provided with air inlet and air outlet, and air inlet is on the side of the test chamber, and air outlet is on the top of the test chamber and deviates to the side away from the air inlet. , the biofilm sample 7 to be tested is placed directly below the gas outlet; the dissolved oxygen microelectrode system includes a dissolved oxygen microelectrode 8, a three-dimensional micromanipulator 9 and a picoammeter 10 with voltage output; A nitrogen cylinder 1 filled with pure nitrogen, an air pump 2 connected to the atmosphere, a flow mete...

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Abstract

The invention discloses a device and method for measuring the diffusion coefficient of dissolved oxygen in a biological membrane. The device comprises a gas delivery device, an intake gas humidification bottle, a biological membrane measuring chamber and a dissolved oxygen microelectrode system. The method for measuring the diffusion coefficient of the dissolved oxygen by utilizing the device comprises the following steps: placing a to-be-measured biological membrane sample into the biological membrane measuring chamber, and introducing nitrogen to reduce the concentration of oxygen in the system to be the minimum; then changing intake gas into oxygen, and meanwhile using a dissolved oxygen microelectrode to record the curve about time-dependent change of the dissolved oxygen in the biological membrane; repeating the test in different positions and different depths of the biological membrane, so as to obtain multiple pieces of sample data; simulating the process through a mathematical model and fitting model data and data measured in the test, and calculating the diffusion coefficient of the dissolved oxygen. According to the invention, the time consumed is short, and precise chemical analysis is not required, so that the measuring result is accurate, the repeatability is good, and the system error is very small.

Description

technical field [0001] The invention relates to the field of electrochemical analysis, in particular to a device and method for measuring the diffusion coefficient of dissolved oxygen in a biofilm. technical background [0002] During aerobic biological reactions, oxygen usually participates in biochemical reactions as an electron donor. Therefore, the concentration of dissolved oxygen in the biofilm will directly affect the reaction rate of the biochemical reaction process. However, due to the very low solubility of oxygen, its transfer rate from the gas phase to the liquid phase and to the biofilm is very small. In the actual treatment process, the mass transfer process of dissolved oxygen often limits the efficiency of biochemical reactions, thus becoming the bottleneck of biochemical processes. The determination of the dissolved oxygen diffusion coefficient in the biofilm is the basis and premise for studying the dissolved oxygen mass transfer process and improving and...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 林君辛青逯鑫淼
Owner 嘉兴华吉环保科技有限公司
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