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Application of highly efficient oil-degrading bacteria and bacteria groups in oily sludge

A technology of degrading bacteria and PetroChina, which is applied in the field of microorganisms, can solve the problems of refractory degradation, less research on microbial degradation and repair, complex components of oily sludge, etc., and achieve the effect of strong degradation ability

Active Publication Date: 2017-09-05
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of oily sludge, which is difficult to degrade, there are relatively few researches on microbial degradation and remediation of oily sludge at home and abroad.

Method used

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  • Application of highly efficient oil-degrading bacteria and bacteria groups in oily sludge
  • Application of highly efficient oil-degrading bacteria and bacteria groups in oily sludge
  • Application of highly efficient oil-degrading bacteria and bacteria groups in oily sludge

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Embodiment Construction

[0023] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0024] This application uses the following methods to screen and confirm the type of bacterial strain, and at the same time provide various excellent properties of the bacterial strain, the specific content is as follows:

[0025] 1 Materials and methods

[0026] 1.1 Oily sludge

[0027] The oily sludge samples come from Bohai Oilfield drilling cuttings, which are taken from the Environmental Protection Technology Service Branch of CNOOC Bohai Company. The water content of the sludge is determined to be 70.55%, the pH value is 6.4, and the salinity is 3.0%.

[0028] 1.2 Medium

[0029] 1.2.1 Enrichment separation medium (g / L) Yeast powder 3, NaCl 5, MgSO 4 ·7H 2 O 0.25, (NH 4 ) 2 SO 4 1,K 2 HPO 4 10,KH 2 PO 4 4. pH 7.2-7.4.

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Abstract

The invention relates to a highly efficient oil-degrading bacteria and bacteria group in oily sludge and its application. The degrading bacteria group includes two kinds of bacteria, one of which is preserved in CGMCC, General Microorganism Center of China Microbiological Culture Collection Management Committee, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Chinese Academy of Sciences, preservation number: CGMCC NO. 8646 and deposit number: CGMCC NO. 8647, the name is Corynebacterium glutamicum (RS1). The present invention also provides a bacterial group consisting of RS3, whose preservation number is CGMCC NO. 8646 and RS1, whose preservation number is CGMCC NO. 8647. All have obvious degradation, and the GC-MS analysis shows that the degradation ability to naphthalene, acenaphthene, chrysene and benzo[b]fluoranthene is relatively strong.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to bacterial strains for degrading petroleum hydrocarbons, in particular to the application of highly efficient petroleum-degrading bacteria and bacterial groups in oily sludge. Background technique [0002] Petroleum is one of the essential energy sources in today's social life and production, and it is also recognized as one of the pollutants that have the most serious impact on the environment. A large amount of oily sludge will be generated during the exploration, development, processing and transportation of petroleum. Oily sludge usually contains alkanes, aromatic hydrocarbons, non-hydrocarbon compounds, and asphaltene, which can pollute the ecological environment and threaten human health through soil and groundwater. Therefore, the safe disposal of oily sludge is a major issue. The treatment and disposal of oily sludge mainly involves the reduction of oil content and the separat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/38C12R1/15
Inventor 刘宪斌任丽君田胜艳赵兴贵
Owner TIANJIN UNIV OF SCI & TECH