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Microbial inoculant, and preparation method and application thereof

A technology of microbial bacterial agents and strains, which is applied in the preparation of microbial bacterial agents and in the field of microbial bacterial agents, can solve the problems of high cost, complicated preparation process of bacterial agents, and long deodorization time, and achieve low cost, good deodorization effect, Effect of short deodorization time

Active Publication Date: 2014-08-13
贵州源熙生物研发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial deodorization technology takes a long time to deodorize, and the preparation process of the bacterial agent is complicated and the cost is high.

Method used

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Examples

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preparation example Construction

[0023] In the embodiment of the present invention, a method for preparing a microbial inoculum is provided, which includes the following steps:

[0024] (a) After solid activation and cultivation of Bacillus brevis 50L-3a, Bacillus agrobacterium and Bacillus amyloliquefaciens strains, the resulting concentrations are all 1-3×10 8 Three bacterial suspensions of CFU / ml;

[0025] (b) Inoculate the three kinds of bacterial suspensions into the first-level seed culture medium with an inoculum of 3-7% by volume, and the concentration is 1-4×10. 10 Three primary seed bacteria suspensions of CFU / ml;

[0026] (c) Mix the three kinds of first-level seed bacteria suspensions in equal volumes, and then transfer them to the second-level seed culture medium with an inoculum of 2-3% by volume, and cultivate to obtain a mixed bacteria concentration of 2-5× 10 11 CFU / ml secondary seed mixed bacterial suspension;

[0027] (d) Transfer the secondary seed mixed bacterial suspension to an inoculum ferment...

Embodiment 1

[0044] Use the following steps to prepare microbial inoculants:

[0045] (a) After solid activation and cultivation of Bacillus brevis 50L-3a, Bacillus agrobacterium and Bacillus amyloliquefaciens strains, wash the bacteria with sterile water containing 0.04% Tween 80 by volume, and mix to obtain a concentration All 1×10 8 Three bacterial suspensions of CFU / ml;

[0046] (b) Inoculate the three bacterial suspensions with the volume percentage of 3% of the inoculum to the first-level seed medium, and the concentration is 1×10. 10 CFU / ml three primary seed bacterial suspensions; among them, the primary seed culture medium is prepared by weighing 10 parts of corn flour, 4 parts of yeast extract, 6 parts of sodium chloride and 1000 parts of distilled water. Mix well, adjust the pH to 7.1, then sterilize at 121°C for 20 minutes, and cool to room temperature to get it;

[0047] (c) Mix the three first-level seed bacteria suspensions in equal volumes, and then transfer them to the second-le...

Embodiment 2

[0054] Use the following steps to prepare microbial inoculants:

[0055] (a) After solid activation and culture of Bacillus brevis 50L-3a, Bacillus agrobacterium and Bacillus amyloliquefaciens strains, rinse the bacteria with sterile water containing 0.05% Tween 80 by volume, and mix to obtain a concentration Both are 2×10 8 Three bacterial suspensions of CFU / ml;

[0056] (b) The three bacterial suspensions were inoculated into the first-level seed medium with an inoculum volume percentage of 5%, and the concentration was 2×10. 10 CFU / ml three primary seed bacterial suspensions; among them, the primary seed culture medium is prepared by weighing 12 parts of corn flour, 5 parts of yeast extract, 8 parts of sodium chloride and 1000 parts of distilled water. Mix well, adjust the pH to 7.2, then sterilize at 121°C for 25 minutes, and cool to room temperature to get it;

[0057] (c) Mix the three first-level seed bacteria suspensions in equal volumes, and then transfer them to the second...

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Abstract

The invention relates to the field of biological products, and particularly relates to a microbial inoculant, and a preparation method and application thereof. The preparation method of the microbial inoculant comprises the following steps: respectively activating Brevibacillus brevis 50L-3a, Brevibacillus agri and Bacillus amyloliquefaciens strains to prepare bacterium suspensions; culturing the three bacterium suspensions to obtain primary seed bacterium suspensions; mixing the three primary seed bacterium suspensions, and culturing to obtain a secondary seed mixed bacterium suspension; and fermenting and culturing until the spore concentration is (2-5)*10<12>CFU / ml and the spores account for more than 85% of the total count in the fermentative bacterium solution, thereby obtaining the microbial inoculant. The preparation method is simple and is low in cost. The microbial inoculant can be used for deodorizing municipal domestic waste, kitchen waste and livestock / poultry manure and preparing organic fertilizers by fermentative rotting, has the advantages of favorable deodorizing effect and short deodorizing time, has the function of preparing organic fertilizers by fermentative rotting, changes wastes into the organic fertilizers, and has important meanings for environmental protection.

Description

Technical field [0001] The present invention relates to the field of biological products, in particular, to a preparation method of a microbial inoculum and the obtained microbial inoculum and application. Background technique [0002] As people's awareness of environmental protection has increased, the requirements for environmental quality have become higher and higher, and they have become more sensitive to pollution caused by malodors. The odor-producing substances not only make people feel uncomfortable and disgusting, but many odorous substances also endanger people’s health and even lives. Therefore, some foreign countries have started research in this area earlier and implemented special legislation on odors. Pollution is separated from air pollution as a kind of public hazard. [0003] The removal of malodorous gas is an extremely important technology, generally including chemical, physical and biological methods. Among them, the microbial deodorization technology in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C05F15/00C12R1/07C12R1/01
CPCY02W30/40
Inventor 周礼红廖瀚峰丁旭
Owner 贵州源熙生物研发有限公司
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