Bacilli for environmental modification of stichopus japonicus aquaculture pond and application thereof

A bacillus and pond technology, which is applied in the field of screening beneficial microorganisms in aquatic products, can solve the problems of drug loss, environmental pollution, and hazards in adjacent waters, and achieve the effects of reducing residues, good growth, and strong ammonia nitrogen and COD degradation capabilities

Active Publication Date: 2014-08-27
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, it is unavoidable to use medicines for frequent diseases. Various antibiotics such as sulfonamides, tetracyclines, penicillins, furans, etc., result in secondary pollution on the breedin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Preparation of PCR template DNA:

[0051] Genomic DNA was extracted by boiling method. After the strain was activated, pick a single colony into 50 μl of sterile distilled water, cook in a boiling water bath at 100°C for 5-10 min, and centrifuge to take the supernatant as PCR template DNA.

[0052] Determination of the 16S rDNA sequence of the strain:

[0053]16SrDNA amplification primers are universal primers for bacterial 16SrDNA, forward primer: 5'-AGAGTT TGA TCC TGG CTC AG-3'(27F), reverse primer: 5'-TAC GGC TAC CTT GTT ACG ACT T-3'(1492R ). According to the PCR system (Niu Yufeng et al., 2009), the strain was amplified by PCR reaction. The PCR product (about 1.5kb) was detected by electrophoresis and sent to Beijing Sanbo Yuanzhi Biotechnology Co., Ltd. for purification and sequencing. The sequencing results were compared with the 16S rRNA sequences of related genus and species in GenBank (http: / / www.ncbi.nlm.nih.gov) using Blast software, and the strains were ...

Example Embodiment

[0059] Example 1:

[0060] Pick a single colony with an inoculating needle and inoculate it into corresponding sterilized Erlenmeyer flasks marked with A1, A2, A3, A4, A5, A6, A7, and A8, respectively. The two Erlenmeyer flasks are not inoculated as blanks. All the Erlenmeyer flasks were placed at 28°C, 160r / min incubator shaker for 5 d. Samples were taken from the corresponding conical flasks and centrifuged at 5000 r / min for 10 min, and the supernatant was taken, and the contents of COD and NH4-N in the supernatant were determined. 100×(1-COD5 / COD0)% and 100×(1-NH4-N5 / NH4-N0)% were used to represent the 5d degradation rates of organic pollutants and ammonia nitrogen in sea cucumber bait by each strain, respectively. The specific values ​​are shown in the table below.

[0061] strain number

[0062] From the experimental results, after 5 days, the 8 screened strains had different degrees of degradation effect on ammonia nitrogen and COD, but the strains had their ...

Example Embodiment

[0065] Example 2:

[0066] Prepare four 250mL conical flasks containing 100mL sterilized bait medium, pick the strain A1 and the two existing Bacillus species (A8, A9) with an inoculating needle, and inoculate them into three sterilized conical flasks respectively. , a Erlenmeyer flask is not inoculated as a blank. The conical flask was placed at 17°C and incubated with shaking at 160 r / min for 5 d. The corresponding conical flask was sampled and centrifuged at 5000 r / min for 10 min, and the supernatant was taken, and the content of COD and NH4-N in the supernatant was determined. 100×(1-COD5 / COD0)% and 100×(1-NH4-N5 / NH4-N0)% were used to represent the 5d degradation rate of organic pollutants and ammonia nitrogen in sea cucumber bait by each strain, respectively. After 5 days, the COD and ammonia nitrogen degradation rates of strain A1 were 57.73% and 38.06%, respectively, the COD and ammonia nitrogen degradation rates of Bacillus A8 on the market were 49.05% and 18.31%, re...

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Abstract

The invention provides the (bacilli sp.) A1 which is stored in a common microorganism center of the China Committee for Culture Collection of Microorganisms of Microbiology Research Institute of China Science Academy which is located at No.3 of yard 1 of the Beichen west road of Chaoyang District of Beijing, and the storage number is CGMCC No. 8686. The provided bacillus strain is original bacilli obtained by being directly separated and screened from aquatic water, and therefore the provided bacillus strain is low in potential fulminant perniciousness to stichopus japonicas and environment ecology when applied to a stichopus japonicus aquaculture pond. The bacillus strain is screened through low temperature and organic degradation capacity to obtain the target bacillus strain, and the target bacillus strain grows well under low temperature and is high in degradation capacity on ammonia nitrogen and COD in bait.

Description

technical field [0001] The invention belongs to the technical field of screening beneficial microorganisms in aquatic products, and in particular relates to a bacillus used for environmental restoration of sea cucumber culture ponds. Background technique [0002] The sea cucumber (Apostichopus japonicus Selenka) aquaculture industry is a new marine aquaculture industry. Since 2002, the sea cucumber aquaculture industry has developed rapidly. In 2006, the national sea cucumber aquaculture area reached 84,000 hectares, the output reached 76,000 tons, and the output value was close to 100 million. billion. However, with the rapid development of its breeding scale, related problems have gradually emerged. At present, most sea cucumber breeding mainly relies on artificial feed, which has poor adhesion, and a large number of artificial attachment bases are specially set up at the bottom of the pond, so a large amount of residual bait and feces will be produced during the breeding...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A61K35/74A61P37/04A23K1/16A23K1/18C12R1/07A23K10/18A23K50/80
Inventor 宋爱环辛美丽刘元文邹琰王英俊孙福新
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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