A kind of azo fluorescent probe for detecting biological hydrogen sulfide and its application

Abstract

The invention provides a fluorescent probe for detecting biological hydrogen sulfide, of which the fluorescence emission wavelength is greater than 500nm. The fluorescent probe has high stability, and thus, can be stored and used for a long time. The fluorescent probe can effectively avoid interference from the biomacromolecule background fluorescence. The fluorescent probe does not have fluorescence itself, has fluorescence only after reaction with H2S, and has high signal to noise ratio for detection and favorable sensitivity. The fluorescent probe has excellent selectivity, and can specifically detect H2S in a complex biological sample. The fluorescent probe has favorable biomembrane permeability, and thus, can be used for specific detection of biological hydrogen sulfide in living cells. The structural formula of the fluorescent probe is disclosed in the specification.

Description

technical field

[0001] The invention belongs to the field of biological detection, and relates to a fluorescent probe for detecting biological hydrogen sulfide and an application thereof. Background technique

[0002] Hydrogen sulfide (H 2 S), a colorless, flammable gas with the smell of rotten eggs, has long been considered a poisonous substance. However, recent studies have shown that mammals can 2 Under the catalysis of S synthetase, a small amount of H is synthesized from homocysteine ​​or cysteine. 2 S, and use it as a signaling molecule to regulate physiological processes such as vasodilation, inflammatory response, and glucose metabolism. h 2 S is the third gas messenger discovered after NO and CO. It can dilate blood vessels by opening ATP-sensitive potassium ion channels in vascular smooth muscle, thereby achieving the effect of lowering blood pressure. h 2 S also has cardiovascular protective effects such as promoting angiogenesis, vascular remodeling, anti-...

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