Method for measuring 14C in biological sample

A biological sample and measurement method technology, applied in the field of carbon content detection, achieves the effects of good measurement accuracy and sensitivity, complicated preparation process, and simple sample preparation method

Inactive Publication Date: 2014-09-24
SUZHOU UNIV
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Problems solved by technology

[0003] At present, my country has not yet issued regulations on biological samples 14 The standard method of C measurement, combined with the EJ / T 1008-96 "in the air 14 C Sampling and Determination" standard method and related liquid scintillation measurement at home and abroad 14 The experimental research progress of C, in this study, the pretreatment process of biological samples was optimized, and the CO with high precision and sensitivity was selected. 2 The direct absorption method was used as a sample preparation method, and a set of liquid scintillation method was established to measure biological samples. 14 C method for our biological samples 14 The development of standard methods of measurement for C, and 14 C Provide technical support for the estimation of the effective dose caused by the human body

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  • Method for measuring 14C in biological sample
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  • Method for measuring 14C in biological sample

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Embodiment 1-4

[0040] By the following methods respectively for green vegetables, tea, radish and corn 14 C content is tested, and described parameter is as shown in table 1, in the gained green vegetables, tealeaves, radish and corn 14 See Table 2 for the specific activity results of C.

[0041] (1) Biological sample pretreatment:

[0042] a. Freeze-drying: wash the biological sample, dry it, cut it into pieces, and weigh the mass as W 1 , after freeze-drying, weighed as W 2 , the formula for calculating the moisture content of biological samples is: ; Use a grinder to crush the above freeze-dried sample into powder, dry at 85 °C for 30 min, and set aside;

[0043] b. Combustion and absorption: Weigh the above-mentioned freeze-dried sample powder, and record the powder weight as W 3 , setting the weight to W 3 The freeze-dried sample powder was placed in a combustion crucible, 50 mL of distilled water was added to the bottom of the crucible, and 15 atm of pure oxygen was filled, deto...

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Abstract

The invention discloses a method for measuring 14C in a biological sample. The method includes the following steps that (1) the biological sample is pretreated, wherein the biological sample is frozen to be in the dry state and burned and absorbs a NaOH solution and finally the biological sample is converted into CaCO3 sediment; (2) a CO2 direct absorption method is used for preparing the sample, wherein CaCO3 powder obtained in the step (1) is dissolved in HCl and a measurement bottle cooled through an ice bath and containing liquid scintillation absorption mixed liquid is used for absorbing generated CO2; (3) a low-background liquid scintillation counter is used for measurement, wherein the sample is measured, so that the counting rate is obtained, and meanwhile the value of the SQP(E) is measured; quenching correction is conducted through an SQP(E) method and the specific activity of 14C in the biological sample is calculated. The method is rapid and accurate in operation and the lower detection limit of 14C specific activity in the biological sample can reach 0.0084 Bq/gC.

Description

technical field [0001] The invention belongs to the field of carbon content detection, in particular to a 14 C measurement method. Background technique [0002] Nuclear power is a new type of energy. After more than half a century of development, nuclear power generation technology has matured, and has been recognized by many countries for its economical and strong competitiveness. At present, there are many coastal and inland nuclear power plants in my country. is constantly being built. While nuclear power solves the energy crisis and brings huge economic benefits for us, there are also some safety hazards and problems. Because nuclear power plants will discharge a certain amount of radionuclides into the surrounding environment during normal operation, of which 14 C is the main radionuclide in the gaseous effluent of nuclear power plants. 14 Because of its long half-life (5730 a), C can widely participate in the metabolic process of organisms along with stable carbon, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01T1/178
Inventor 张友九毕梦乔江华朱琳张云艳
Owner SUZHOU UNIV
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