Systems and methods for visualizing ablated tissue
Tissue, vascular technology, in the field of systems and methods for visualizing ablated tissue
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[0109] experiment procedure
[0110] Experiments were performed using animal hearts to compare the validity of NADH-recorded imaging using the present disclosure with triphenyltetrazolium chloride (TTC) staining comparison. As will be described in more detail below, imaging using NADH recordings performed as well as TTC staining. Importantly, it is done in living tissue and does not require any additional processing time or the use of dyes to achieve the same performance.
[0111] animal process
[0112] Ex vivo experiments were performed using dissected bloodless hearts of rats (200-300 g Sprague-Dawley, n=8) and rabbits (2.5-3.5 Kg New Zealand White, n=3). Animals were heparinized and anesthetized using standard procedures. The heart was then dissected, the aorta was cannulated and infused with oxygenated buffered Tyrode's solution at room temperature Langendorff at constant pressure (50 mmHg). The heart was placed on top of a grounding pad and immersed in 37°C Tyrode'...
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