Application of stc2 gene as a molecular marker for colorectal cancer liver metastasis

A colon cancer and gene technology, applied in the biological field, can solve the problems of unfound sensitivity, specificity, etc.

Inactive Publication Date: 2016-05-11
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of markers related to tumor metastasis of colorectal cancer, although a lot of research has been done, a marker with high enough sensitivity and specificity that is suitable for early diagnosis has not yet been found.

Method used

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  • Application of stc2 gene as a molecular marker for colorectal cancer liver metastasis
  • Application of stc2 gene as a molecular marker for colorectal cancer liver metastasis
  • Application of stc2 gene as a molecular marker for colorectal cancer liver metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Discovery of molecular marker gene STC2

[0033] 1. Expression profiling microarray screening of differentially expressed genes in colorectal cancer liver metastases

[0034] 1. Extract RNA

[0035] There were 6 cases of colorectal cancer, including 2 males and 1 female in the colorectal cancer liver metastasis group, with a median age of 54.67 years; 2 males and 1 female in the colorectal cancer non-hepatic metastasis group, with a median age of 68.67 years. As shown in Table 1 below, the sample name is indicated by the hospitalization number of the patient, with two digits hidden in the middle.

[0036] Table 1 is the colorectal cancer cases

[0037]

[0038] The RNA of the cancer tissues of the 6 colorectal cancer patients mentioned above was extracted, and the RNA quality inspection results of the 6 samples are shown in Table 2 below. In this study, NanodropND-1000 was used to measure the absorbance at 260nm and 280nm to determine the concentration ...

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Abstract

The invention discloses application of an STC2 gene as a colorectal cancer liver metastasis molecular marker. The invention provides a kit for identifying or assisting in identifying whether a to-be-detected colorectal cancer patient has liver metastasis or not. The kit comprises a reagent for detecting expression quantity of the STC2 gene and a carrier for recording the following judgment standards: if the expression quantity of the STC2 gene in a cancer tissue of the to-be-detected colorectal cancer patient is higher than that of the STC2 gene in the cancer tissue of the diagnosed non-liver metastasis colorectal cancer liver patient, and the two have remarkable difference, the to-be-detected colorectal cancer patient has or is candidate to have liver metastasis. Experiments in the invention prove that expression of the STC2 in primary lesion of patients in the colorectal cancer liver metastasis group is remarkably higher than that in a non-liver metastasis group, and in-vitro functional experiment result at a later period indicates that STC2 possibly promotes tumor metastasis invasion ability to participate in a colorectal cancer liver metastasis process, so that clue is provided for further finding the colorectal cancer liver metastasis molecular marker.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an application of STC2 gene as a molecular marker of colorectal cancer liver metastasis. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive tract, and its incidence is second only to gastric cancer and esophageal cancer. The most important factor affecting the prognosis of patients with colorectal cancer is cancer metastasis, among which liver metastasis is the most common. According to statistics, 20%-40% of patients with colorectal cancer have developed liver metastases when they are diagnosed, and the incidence of metachronous liver metastases is as high as 50%. The average survival period of patients with liver metastases without treatment is 16-18 months, while the survival period of patients with extensive metastasis is only 3-5 months. The vast majority of patients with liver metastases cannot be radically resected due to factors suc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158
Inventor 谢伟杨倩冯茂辉马翔陈双倩王国洲李华驰
Owner WUHAN UNIV
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