Double-screw micro-fluidic chip

A microfluidic chip and double helix technology, applied in the field of clinical medicine, can solve the problems of many operation procedures, complex chip preparation and high cost, and achieve the effects of high enrichment rate, improved accuracy and precision, and low cost.

Active Publication Date: 2014-10-22
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The overall preparation of the chip is complicat...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Microfluidic device for separating leukocytes from blood

[0028] (1) The blood sample is gently inverted back and forth to make it evenly mixed;

[0029] (2) Dilute the blood 10 times with PBS, draw it with a 5ml syringe; then the syringe is integrally fixed on the constant flow pump, the outlet of the syringe is connected to the interface of the microfluidic chip device, and the flow rate of the blood pump is 20ml / h; At the same time, the same samples were loaded onto glass slides, spread evenly, and air-dried;

[0030] (3) After the blood passes through the microfluidic device, the Giemsa dye solution is automatically injected along the channel at a rate of 1ml / h, and the membrane attached to the target cell is stained for 10 minutes; at the same time, the Giemsa dye solution is attached to the On the cell slide of the blood sample, stain for 10 minutes;

[0031] (4) After the staining is completed, the PBS washing solution is automatically injected alon...

Embodiment 2

[0034] Example 2: Microfluidic device for separating leukocytes in pleural effusion

[0035] (1) The pleural effusion sample is gently inverted back and forth to make it evenly mixed;

[0036] (2) Dilute the pleural effusion 100 times with PBS, draw it with a 5ml syringe; then the syringe is fixed on the constant flow pump as a whole, the outlet of the syringe is connected to the interface of the microfluidic chip device, and the flow rate of the pleural effusion pump is 20ml / h; at the same time, the same sample was loaded onto a glass slide, spread evenly, and air-dried;

[0037] (3) After the pleural effusion passes through the microfluidic device, the Giemsa dye solution is automatically injected along the channel at a rate of 1ml / h to stain the membrane attached to the target cells for 10 minutes; Stain for 10 minutes on cell slides containing pleural effusion samples;

[0038] (4) After the staining is completed, the PBS washing solution is automatically injected along...

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Abstract

The invention relates to a double-screw micro-fluidic chip which comprises a chip inlet, a double-screw chip, an erythrocyte outlet, a leukocyte outlet, a fluid outlet and a cell filter membrane, wherein the chip is composed of two-screw-type channels, the two-screw-type channels are respectively punched along two tangent line directions of two mutually parallel chips to form two parallel reversed openings, one opening is connected with the chip inlet, and the other opening is respectively connected with the erythrocyte outlet, the leukocyte outlet and the fluid outlet. The double-screw micro-fluidic chip is capable of automatically feeding, dyeing and washing a sample, and automatically separating and enriching leukocytes in blood or/and pleural effusion of a patient suffering from leucocythemia at a high flux, thereby greatly improving diagnosis accuracy and precision; meanwhile, the double-screw micro-fluidic chip can be further used for separating in-vivo diseased leukocytes of the patient, and has a good treatment effect.

Description

technical field [0001] The invention relates to the field of clinical medicine, and relates to a chip for disease detection and treatment, in particular to a microfluidic chip for leukemia detection and treatment. Background technique [0002] Leukemia is a malignant clonal disease of hematopoietic stem cells. Clonal leukemia cells proliferate and accumulate in bone marrow and other hematopoietic tissues due to mechanisms such as uncontrolled proliferation, differentiation disorder, and apoptosis inhibition, and infiltrate other tissues and organs, while normal hematopoiesis is inhibited. Clinically, different degrees of anemia, hemorrhage, infection, fever, enlargement of liver, spleen, lymph nodes and bone pain can be seen. According to reports, the incidence of leukemia in various regions of my country occupies the sixth place among various tumors. Children and adolescents with acute leukemia often have a sudden onset. Common initial symptoms include fever, progressive...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N1/40G01N1/31B01L3/00
Inventor 蒋兴宇刘野孙佳姝王纪东
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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