Ovum and embryo treating fluid and preparation method thereof
A technology for treating liquid and embryos, which is applied in the field of egg and embryo processing liquid and its preparation, can solve the problems of poor performance of solution effectiveness and high cost, and achieve the effect of inhibiting the growth of bacteria and good thermal stability
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[0023] The present invention will be further described below by way of examples.
[0024] 1. Formula and preparation method of egg and embryo treatment solution
[0025] Table 1 The formula of ovum and embryo treatment solution
[0026]
[0027] The preparation method of the ovum and embryo treatment liquid of the present invention comprises the following steps:
[0028] (1) Preparation of stock solution for egg and embryo treatment solution
[0029] a. First weigh each component according to the proportion and place them separately;
[0030] b. First, dissolve the weighed components (except bactericide, selective indicator, sodium bicarbonate) in ultrapure water. During the dissolution process, follow the principle of first solid and then liquid, NaHCO 3 the principle of last access;
[0031] c, add weighed bactericide, indicator and NaHCO selectively in the solution obtained in step b 3 ;
[0032] d. Detect the osmotic pressure and pH value of the solution. If the p...
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