Capripoxvirus (CPV) Taqman-MGB (minor groove binder) probe multiple real-time fluorescence quantitative PCR (polymerase chain reaction) detection primer, kit and detection method

A technology of real-time fluorescence quantification and sheep pox virus, which is applied in the field of animal virus molecular biology inspection, can solve the problem of no inactivation effect, and achieve the effect of simple identification
CN104152584AActive Publication Date: 2014-11-19重庆海关技术中心 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
重庆海关技术中心
Publication Date
2014-11-19

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Abstract

The invention discloses a capripoxvirus (CPV) Taqman-MGB (minor groove binder) probe multiple real-time fluorescence quantitative PCR (polymerase chain reaction) detection primer, a kit and a detection method. The multiple Taqman-MGB real-time fluorescence quantitative PCR detection method can be used for rapid detection of Lumpy Skin Disease Virus (LSDV), Goatpox Virus (GTPV) and Sheeppox Virus (SPPV). According to the method, on the basis of conserved domains of target sequences of the LSDV, the GTPV and the SPPV, a pair of primer and three Taqman-MGB probes are designed. The method only needs two step amplification method and simple reaction conditions for fast, efficient, specific and highly-sensitive detection of an objective target sequence, is simple in operation, does not need expensive equipment and reagents, has no technical requirement on operators, is low in detection cost and short in testing time, and can avoid cross contamination caused by agarose electrophoresis so as to improve the detection accuracy and reliability.
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Description

technical field

[0001] The invention relates to the field of animal virus molecular biology testing methods, in particular to a primer, method and kit for multiplex real-time fluorescence quantitative PCR detection of capipoxvirus Taqman-MGB probes. Background technique

[0002] Capripoxvirus (CPV) includes Goatpox Virus (GTPV), Sheep Pox Virus (SPPV) and Lumpy Skin Disease Virus (LSDV), which can cause goats, Extensive nodules and edema on the skin and organ surfaces of sheep and cattle. The milk production of sick animals is drastically reduced, and the quality of fur is greatly reduced, causing huge economic losses. Goat pox, sheep pox caused by sheep pox virus, also known as sheep "smallpox", is an acute, febrile, contact infectious disease of sheep. Sheep pox and goat pox are listed as a first-class infectious disease in my country, which has a great impact on animal husbandry. According to the difference in virulence of different strains, the lethality rate of suscep...

Claims

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