Preparation method of targeting octreotide-modified chitosan molecular beacon nanocomposite for lung cancer diagnosis

A nanocomposite and molecular beacon technology, applied in the field of preparation of chitosan molecular beacon nanocomposite, can solve the problems of poor specificity or stability, harsh process conditions, complicated preparation methods, etc., and achieve high specificity. , High transfection efficiency and good stability

Inactive Publication Date: 2016-08-10
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of preparation method of the chitosan molecular beacon nanocomposite that is used for the targeting octreotide modification of lung cancer diagnosis, solves the complicated preparation method of the medicine or the reagent that is used for lung cancer diagnosis in the prior art The conditions are harsh and its targeting, specificity or stability is not good, and it can only diagnose the problems of middle and advanced lung cancer

Method used

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  • Preparation method of targeting octreotide-modified chitosan molecular beacon nanocomposite for lung cancer diagnosis
  • Preparation method of targeting octreotide-modified chitosan molecular beacon nanocomposite for lung cancer diagnosis
  • Preparation method of targeting octreotide-modified chitosan molecular beacon nanocomposite for lung cancer diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Mix chitosan nanometers and molecular beacons in PBS buffer at pH 6.0 according to the mass ratio of 7:1, vortex and shake for 60s, and stand at room temperature for 60min to obtain chitosan molecular beacon nanocomposites ; Add 1.0% glutaraldehyde solution dropwise to the chitosan molecular beacon nanocomposite, the mass ratio of the glutaraldehyde solution to the chitosan molecular beacon nanocomposite is 1000:1, mix by stirring for the first time Carry out the reaction for 1.5h, the stirring speed for the first time is 150rpm, and remove unbound glutaraldehyde by dialysis for the first time through a microdialysis column after the reaction; Add octreotide in a ratio of 1:1, carry out the second stirring and mixing reaction for 4 hours, the second stirring speed is 150rpm, after the reaction, the unbound octreotide is removed through the second dialysis column for 12 hours, and the target particle size of 45nm~55nm is obtained. Octreotide-modified chitosan molecular b...

Embodiment 2

[0040] Example 2: The difference from Example 1 is that the mass ratio of chitosan nanometer and molecular beacon is 10:1, and finally a chitosan molecular beacon nanocomposite modified with targeting octreotide with a particle size of 40nm-55nm is obtained thing.

Embodiment 3

[0041] Example 3: The difference from Example 1 is that the mass ratio of chitosan nanometer and molecular beacon is 8:1, and finally a chitosan molecular beacon nanocomposite modified with targeting octreotide with a particle size of 45nm-60nm is obtained thing.

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Abstract

The invention discloses a targeted somatostatin analog octreotide (OCT)-modified chitosan (CS) molecular beacon (MB) nanocomposite for lung cancer diagnosis. The preparation method comprises the following steps: synthesizing a chitosan molecular beacon nanocomposite by virtue of a self-assembly method, and synthesizing the targeted octreotide-modified chitosan miR-155 (microRNA-155) molecular beacon nanocomposite by virtue of a chemical reaction by taking glutaraldehyde as a crosslinking agent. The targeted octreotide-modified chitosan molecular beacon nanocomposite can be used for dynamically monitoring the occurrence and development process of lung cancer according to fluorescence intensity, can be applied to imaging lung carcinoma cell and lung cancer bearing cancer mouse living animals, has the characteristics of high targeting property, high transfection efficiency, high specificity, high stability, low background and the like and provides new thoughts, new methods and new technologies for early diagnosis of lung cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing a targeting octreotide-modified chitosan molecular beacon nanocomposite for lung cancer diagnosis. Background technique [0002] Lung cancer is a malignant tumor that seriously threatens human health. Its morbidity and mortality are increasing year by year. Early diagnosis and early treatment are the key to improving the prognosis of patients. However, lung cancer patients often have no obvious symptoms in the early stage of the disease. best time for treatment. Therefore, starting from the "origin" of lung cancer and exploring new methods for efficient and specific early diagnosis will have a profound impact on the prevention, diagnosis and treatment of lung cancer. [0003] The theory of cancer stem cells holds that there is a group of very small number of tumor cells in tumor tissue cells, called tumor stem cells, and all tumor cells are differentia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 陈正堂孙建国朱海振姚权安江宏韩静李雪涛朱聪慧江飞龙陈光朋彭丽娜
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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