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Stable troponin I detection reagent

A troponin and detection reagent technology, applied in the field of troponin I detection reagents, to achieve the effects of improving analytical sensitivity, accuracy and stability, improving stability and anti-interference ability

Active Publication Date: 2014-12-03
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunoturbidimetry has the advantages of low price, accurate and fast results, good repeatability, and automatic batch analysis. It is widely used in clinical practice, but it also has some disadvantages, such as poor reagent stability and sensitivity to be improved.

Method used

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  • Stable troponin I detection reagent
  • Stable troponin I detection reagent

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0025] HEPES (hydroxyethylpiperazine ethylsulfuric acid) buffer 0.05mol / L

[0026] NaCl (sodium chloride) 10mmol / L

[0027] polyethylene glycol 6000 3g / L

[0028] Alkyl glycoside 3g / L

[0029] Preservative NaN 3 0.5g / L;

[0030] 2) The components of reagent R2 are:

[0031] HEPES (hydroxyethylpiperazine ethylsulfuric acid) buffer 0.05mol / L

[0032] Goat anti-human TnI antibody coated latex particles 3.5% (V / V)

[0033] polyethylene glycol 6000 3g / L

[0034] Alkyl glycoside 3g / L

[0035] Preservative NaN 3 0.5g / L.

[0036] 3) The method of using the reagents in this example:

[0037] The troponin I detection reagent described in this embodiment adopts a fully automatic biochemical analyzer with dual reagent functions during use, such as Toshiba 40 fully automatic analyzer, etc., and R1 and R2 are placed in the corresponding ratio of 3:1. On the reagent position, place distilled water, standards and samples on the corresponding positions of the sa...

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Abstract

The invention provides a stable troponin I detection reagent. The stable troponin I detection reagent comprises a reagent R1 and a reagent R2 in a volume ratio of 3 to 1, wherein the reagent R1 comprises the following ingredients: 0.05mol / L of HEPES buffer solution, 10mmol / L of NaCl, 3g / L of polyethylene glycol 6000, 3g / L of alkyl glycoside and 0.5g / L of antiseptic NaN3; the reagent R2 comprises the following ingredients: 0.05mol / L of HEPES buffer solution, 3.5 percent of goat anti-human TnI antibody coated latex particles, 3g / L of polyethylene glycol 6000, 3g / L of alkyl glycoside and 0.5g / L of antiseptic NaN3. The stable troponin I detection reagent is good in accuracy and stability, low in price and convenient to use and capable of completely meeting the clinical requirement.

Description

technical field [0001] The invention relates to a stable troponin I detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Troponin is a structural protein that makes up striated muscle thin filaments, and its subunits I, T, and C form a complex. Plays an important regulatory role in the process of muscle contraction and relaxation. Among them, troponin I (cTnI) has been proved to be one of the most specific and sensitive serum markers of myocardial injury after extensive research. The content in the blood of normal people is generally lower than 0.3μg / L. When the integrity of the myocardial cell membrane is damaged due to ischemia or hypoxia. Free cTnI can quickly pass through the cell membrane and enter the bloodstream. Therefore, in the early stage of the disease. Rapid, sensitive and accurate determination of cTnI in human blood and its changing trend has important clinical significance for the diagnos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31
CPCG01N21/31G01N33/544G01N33/68
Inventor 谭柏清李志明甘宜梧王绮李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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