Automatic digital slide focusing device and method based on microscope
A digital slicing and auto-focusing technology, applied in microscopes, installations, optics, etc., can solve problems such as focus oscillations
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Embodiment 1
[0052] Such as figure 1 As shown, the microscope-based digital slice automatic focusing device provided in this embodiment includes a trinocular microscope 1 and a computer 8. An automatic object loading platform 2, a scanning eyepiece 3 and a focusing knob 4 are installed on the trinocular microscope 1. The automatic object loading platform 2 is detachably installed on the trinocular microscope 1, and is used for moving along the Z-axis direction, and the repeated positioning accuracy along the Z-axis direction is 0.09um. The automatic object loading platform 2 is fixedly connected with a focusing sleeve 6; A high-speed area-scan digital camera 7 is installed on the scanning eyepiece 3, and is connected with a computer 8 by a USB3.0 connection line. The frame rate of the high-speed area-scan digital camera 7 is 120 frames; 6 is connected with the Z-axis motor 5; the Z-axis motor 5 is connected to the automatic loading platform controller 9 through the RS232 connection line, ...
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Abstract
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Application Information
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