Culture medium for anti-glufosinate-ammonium alfalfa plants and soil screening method

A technology of screening methods for alfalfa, applied in botany equipment and methods, horticultural methods, plant genetic improvement, etc., can solve problems such as screening methods for glufosinate-resistant alfalfa plants that have not been disclosed or published, and achieve rapid results Effect

Inactive Publication Date: 2014-12-10
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, before the application of the present invention, the screening method of glufosinate-resistant alfalfa plants has not been disclosed or published in China

Method used

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  • Culture medium for anti-glufosinate-ammonium alfalfa plants and soil screening method
  • Culture medium for anti-glufosinate-ammonium alfalfa plants and soil screening method
  • Culture medium for anti-glufosinate-ammonium alfalfa plants and soil screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: a kind of culture medium screening method of glufosinate-resistant alfalfa plant is characterized in that comprising the steps:

[0053] (1) MS medium to germinate seeds

[0054] Select alfalfa seeds; prepare MS culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 100% alcohol for 0.5 min; soak in 2% sodium hypochlorite solution for 15 min; ddH 2 O Wash the seeds 10 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 22°C, 18 hours of light, 6 hours of darkness to germinate; the radicle breaks through the testa For germination, the 6th day, the average germination rate reaches more than 50%, stand-by;

[0055] (2) Dose screening

[0056] Glufosinate-ammonium sets 9 treatments, the concentrations are 0mg / L, 1mg / L, 2mg / L, 3mg / L, 5mg / L, 8mg / L, 10mg / L, 12mg / L, 15mg / L, the configurations are different Concentration of glufos...

Embodiment 2

[0065] Embodiment 2: a kind of culture medium screening method of glufosinate-resistant alfalfa plant is characterized in that comprising the steps:

[0066] (1) MS medium to germinate seeds

[0067] Select alfalfa seeds; prepare MS medium culture plates for later use; carry out seed disinfection under sterile conditions: soak seeds in 75% alcohol for 1 min; soak in 2% sodium hypochlorite solution for 10 min; ddH 2 O Wash the seeds 5 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 20°C, 16 hours of light, 8 hours of darkness to germinate; the radicle breaks through the seed coat For germination, the 5th day, the average germination rate reaches more than 50%, stand-by;

[0068] (2) Dose screening

[0069] Glufosinate-ammonium sets 9 treatments, the concentrations are 0mg / L, 1mg / L, 2mg / L, 3mg / L, 5mg / L, 8mg / L, 10mg / L, 12mg / L, 15mg / L, the configurations are different Concentration of...

Embodiment 3

[0078] Embodiment 3: a kind of medium screening method of glufosinate-resistant alfalfa plant is characterized in that comprising the steps:

[0079] (1) MS medium to germinate seeds

[0080] Select alfalfa seeds; prepare MS medium culture plates for use; carry out seed disinfection under sterile conditions: soak seeds in 60% alcohol for 2 minutes; soak in 2% sodium hypochlorite solution for 5 minutes; ddH 2O Wash the seeds 3 times; spread the sterilized seeds evenly on the MS medium with tweezers, seal them with a parafilm and place them in an artificial incubator, 18°C, 0h light, 24h dark to germinate; the radicle breaks through the testa For germination, the 3rd day, the average germination rate reaches more than 50%, stand-by;

[0081] (2) Dose screening

[0082] Glufosinate-ammonium sets 9 treatments, the concentrations are 0mg / L, 1mg / L, 2mg / L, 3mg / L, 5mg / L, 8mg / L, 10mg / L, 12mg / L, 15mg / L, the configurations are different Concentration of glufosinate-ammonium medium, ea...

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Abstract

The invention mainly relates to a screening method of anti-glufosinate-ammonium alfalfa plants, in particular to an anti-glufosinate-ammonium germplasm screening method. A screening method for a culture medium for anti-glufosinate-ammonium alfalfa plants is mainly characterized by comprising the following steps: (1) germinating seeds by the MS (Murashige and Skoog) culture medium; (2) screening the dose; (3) inducing alfalfa seeds through ethylmethane sulfonate median lethal dose; (4) screening the anti-glufosinate-ammonium alfalfa plants; and (5) screening resistant plants again. Compared with the prior art, the method has the advantages of being simple and easy to implement, taking effect quickly, and being capable of obtaining the herbicide resistant alfalfa individual plant in a short term. The method adopts a mutation measure, utilizes a chemical mutagen EMS (ethylmethane sulfonate) to induce the alfalfa seeds to cause point mutation, can screen the herbicide resistant alfalfa individual plant by the culture medium and the soil screening method, can provide a new germplasm resource for conventional breeding and can provide a reference for breeding of herbicide resistant agents of other plants.

Description

technical field [0001] The invention mainly relates to a screening method for glufosinate-resistant alfalfa plants. It specifically relates to a screening method for germplasm resistant to glufosinate-ammonium. Background technique [0002] Alfalfa (Medicago sativa L.) has the characteristics of high quality, high yield, high feed value (crude protein content 21%), good palatability, and wide range of uses. It has the functions of preserving fertilizer and water and soil, has edible value, and has a good prospect for industrialization. There are many kinds of weeds in alfalfa field, both dicotyledonous weeds and monocotyledonous weeds, among which the damage of dicotyledonous weeds is the most serious. Weeds compete with alfalfa for sunlight, water, nutrients and space, and will also spread diseases and insect pests to alfalfa, which has a serious adverse effect on the quality and yield of alfalfa. Statistics from the Food and Agriculture Organization of the United Nations...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H4/00
Inventor 张吉宇霍雅馨段珍狄红艳骆凯吴凡
Owner LANZHOU UNIVERSITY
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