Sequential lateral flow capillary device for analyte determination
A flow capillary and capillary technology, applied in the field of analyte detection, can solve problems that prevent the execution of real sequential reactions, affect the repeatability and accuracy of results, and affect the duration of a given step.
Active Publication Date: 2014-12-31
REALBIOTECH
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[0016] The second limitation is that the liquid must be added at the same time
The mixing of two liquids, and as described above, leads to unpredictable results, adversely affects the duration of a given step, prevents the performance of a true sequential reaction, and affects both the repeatability and accuracy of results
[0017] A third limitation is that the teaching of EP1044372 can lead to the formation of multiple capillary paths
Consequently, liquid leaks away from all liquid-holding regions through any alternate capillary path, filling the lateral-flow capillary device with liquid and rendering experimental results useless
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[0085] The present invention is a lateral flow capillary device and methods of using the same. The present invention allows the performance of efficient and reproducible multi-step reactions such as multi-step specific binding assays for serological testing.
[0086] In the specification, the embodiments relate to analytical methods for the detection of analytes, which are biomarkers such as antigens, antibodies, metabolites, poisons or other detectable material from human or other living sources such as blood, urine, Tissue, or other detectable material from non-living sources such as environmental sources such as water, soil or sewage. In the specification, the embodiments relate to binding an analyte to an anti-reactant immobilized at a reaction zone on a capillary flow matrix, said anti-analyte together with the analyte constituting a specific binding pair such as an antibody, antigen, DNA or other specific A sex binding pair (sbp) member, and the bound analyte is then de...
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Disclosed is a lateral flow capillary device (24) and uses thereof comprising a unipath capillary flow matrix (18) and a plurality of reservoirs ((32a)(32b)(32c)) each in fluid communication with the capillary flow matrix (18). The device includes one or more pressure delivery systems ((36a)(36b)(36c)(38a)(38b)(38c)) configured to urge capillary flow matrix (18) toward a portion of the housing (40) in which it resides, such that a substantially uniform pressure is applied over a portion of the capillary flow matrix (18).
Description
technical field [0001] The present invention relates to the field of biology and analyte detection from environmental and biological fluids, mainly at the point of care, and more particularly to improved lateral flow capillary devices and methods for using lateral flow capillary devices such as Methods for performing specific binding assays. Background technique [0002] The use of specific binding assays is of great value in a variety of clinical and other applications, see eg PCT patent application US2004 / 031220 published as WO 2005 / 031355. Specific binding assays involve the detection and preferably quantitative determination of an analyte in a sample, wherein the analyte is a member of a specific binding pair consisting of a ligand and a receptor. Ligand and receptor constituting a specific binding pair are related in that receptor and ligand specifically bind to each other. Specific binding assays include immunoassays involving reactions between antibodies and antigen...
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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/5023B01L2200/026B01L2200/12B01L2200/16B01L2300/0825B01L2400/0406G01N1/28B01L3/50273
Inventor E·西淼诺夫A·加吉尔A·莱茵哈茨S·阿拉吉姆
Owner REALBIOTECH