Method for quickly producing Chinese fir seedlings through micro-cutting

Abstract

A method for quickly producing Chinese fir seedlings through micro-cutting comprises steps as follows: taking Chinese fir tissue culture cluster seedlings for subculture proliferation strong seedling culture to obtain adventitious buds; transferring the adventitious buds to a plastic greenhouse for seedling exercising to obtain rootless seedlings, performing pretreatment on the rootless seedlings, soaking the pretreated rootless seedlings in a rooting agent solution, transplanting the rootless seedlings to seedling culture bags, managing the seedlings after cutting, and inducing the seedlings to root to obtain asexually-reproduced Chinese fir seedlings. With the adoption of the method, the seedling forming time can be shortened, the strong degree of the seedlings can be increased, the production cost is also reduced, mass production of excellent clone seedlings of Chinese fir is facilitated, and the method is an economical, practical and efficient Chinese fir seedling production technology.

Description

technical field [0001] The invention relates to a method for micro-cuttage transplantation in the field of plant regeneration technology, in particular to a method for rapidly producing Chinese fir seedlings by micro-cuttage, and belongs to the field of plant biotechnology. Background technique [0002] Chinese fir is a unique native tree species in my country and one of the most important commercial timber tree species in the south of the Yangtze River. It has the advantages of fast growth, high yield, good material and wide application. It is widely used in Guizhou, Hunan, Guangxi, Guangdong, Fujian and Jiangxi. Widely cultivated in other provinces. As one of the central producing areas of Chinese fir, Guangxi has a good provenance reserve, but its cultivation method is based on seedling afforestation for seed propagation. Due to the influence of climate and other factors, the output of Chinese fir seed gardens fluctuates greatly, often facing the problem of seedlings. lac...

AI Technical Summary

Problems solved by technology

[0005] Chinese patent "A method for in vitro rooting culture of fir clones" (CN102217539), after the fir explants are induced to differentiate into adventitious buds, they are transferred to MS medium for subculture and culture, and the subculture formula is 3 / 4MS +6BA0.3mg / L+ sucrose 3%; when the adventitious buds elongate to 2-3cm, transfer to the rooting induction medium, the formula of the rooting medium is 1 / 4MS, and the plant growth regulator contains IBA 0.05-0.2mg / L, NAA 0.05-0.4mg / L, the rooting rate of Chinese fir tissue culture seedlings in the bottle can reach up to 100%, but the rooting is not neat; For 70% of the seedling growth medium, no light medium is used, and the root system is not developed enough

This report helps to partially solve the problem of transplanting and rooting test-tube seedlings that cannot take root on the rooting medium in the bottle, but does not solve the problems of improving the rooting speed of Chinese fir, shortening the seedling cultivation period of Chinese fir seedlings, and reducing the cost of seedling cultivation, and the seedling cultivation substrate is still Yellow core soil cannot meet the requirements of modern light substrate seedling raising technology and the requirements of automatic substrate filling equipment, which affects the production efficiency of seedlings

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