Method for quickly producing Chinese fir seedlings through micro-cutting

A technology of micro-cutting and Chinese fir, which is applied in the field of plant biology, can solve the problems of increasing the rooting speed of Chinese fir, shortening the seedling raising cycle of Chinese fir seedlings, affecting the production efficiency of seedlings, etc., and achieves shortening the seedling raising cycle, good fertilizer retention performance, and light weight. Effect

Inactive Publication Date: 2015-01-07
GUANGXI UNIV
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Problems solved by technology

[0005] Chinese patent "A method for in vitro rooting culture of fir clones" (CN102217539), after the fir explants are induced to differentiate into adventitious buds, they are transferred to MS medium for subculture and culture, and the subculture formula is 3/4MS +6BA0.3mg/L+ sucrose 3%; when the adventitious buds elongate to 2-3cm, transfer to the rooting induction medium, the formula of the rooting medium is 1/4MS, and the plant growth regulator contains IBA 0.05-0.2mg/L, NAA 0.05-0.4mg/L, the rooting rate of Chinese fir tissue culture seedlings in the bottle can reach up to 100%, but the rooting is not neat; For 70% of the seedling growth medium, no light me

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  • Method for quickly producing Chinese fir seedlings through micro-cutting
  • Method for quickly producing Chinese fir seedlings through micro-cutting
  • Method for quickly producing Chinese fir seedlings through micro-cutting

Examples

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Embodiment 1

[0026] The method for fast production of Chinese fir seedlings by micro-cutting of the present invention comprises the following steps:

[0027] (1) Subculture proliferation and strong seedling cultivation: take fir tissue culture clustered seedlings and inoculate them into MS strong seedling medium, under the condition that the culture temperature is 23-26°C, the light intensity is 1800-2000lux, and the light time is 12 hours / day Proliferation and cultivation under low temperature for 40 days can obtain clustered sprouts. The MS strong seedling medium is 6-BA containing 0.6mg / L, 0.3mg / L IBA, 1.0mg / L KT, 30g / L sucrose, 5g / L agar and 0.5g / L activated carbon. The pH of the medium was 5.8.

[0028] (2) Seedling hardening: When the adventitious buds are cultivated to 4cm-5cm in the tissue culture room, they are moved to a plastic greenhouse for hardening. There are ventilation devices around the plastic greenhouse, and the seedlings are hardened under natural light for 15-20 days...

Embodiment 2

[0033] The 2nd example of the method for the rapid production of Chinese fir seedlings by micro-cutting of the present invention, except that the following operation steps and control technical conditions are different, other steps are the same as embodiment 1.

[0034] Soaking time of rooting agent: After subculture and multiplication of aseptic Chinese fir seedlings, take out 4-5 cm rootless test-tube seedlings after hardening, wash the medium, cut off callus, and soak in 1‰ carbendazim 10min, then soak the 0.8cm-1.0cm part of the base of the seedlings in the rooting agent solution, the rooting agent contains 50mg / L IBA and 25mg / L NAA, soak the rootless test tube seedlings in the rooting agent for 5 minutes and 15 minutes respectively , 25 minutes, with yellow heart soil as the substrate. After 50 days of transplanting, the effect of soaking for 15 minutes is the best, the rooting rate reaches 80%, and the chlorophyll content is 1.68 mg / g; the rooting rates of soaking for 5 ...

Embodiment 3

[0036] The 3rd example of the method for the rapid production of fir seedlings by micro-cutting of the present invention, except that the following operating steps and control technical conditions are different, other steps are the same as in Example 1.

[0037] Optimizing the type and concentration of rooting agent, the type of substrate and the ratio: after the aseptic seedlings of Chinese fir were subcultured and multiplied, the 4-5 cm rootless test tube seedlings were taken out after hardening, the medium was washed, and the callus was cut off. Soak in 1‰ carbendazim for 10 minutes, and then soak the 0.8cm-1.0cm part of the seedling base in the rooting agent solution. Orthogonal design was used to set different concentrations of IBA and NAA and different substrate types, and the rooting conditions and chlorophyll content of seedlings were measured 50 days after transplanting. The results in Table 2 show that the influence of the substrate on the micro-cuttage rooting rate,...

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Abstract

A method for quickly producing Chinese fir seedlings through micro-cutting comprises steps as follows: taking Chinese fir tissue culture cluster seedlings for subculture proliferation strong seedling culture to obtain adventitious buds; transferring the adventitious buds to a plastic greenhouse for seedling exercising to obtain rootless seedlings, performing pretreatment on the rootless seedlings, soaking the pretreated rootless seedlings in a rooting agent solution, transplanting the rootless seedlings to seedling culture bags, managing the seedlings after cutting, and inducing the seedlings to root to obtain asexually-reproduced Chinese fir seedlings. With the adoption of the method, the seedling forming time can be shortened, the strong degree of the seedlings can be increased, the production cost is also reduced, mass production of excellent clone seedlings of Chinese fir is facilitated, and the method is an economical, practical and efficient Chinese fir seedling production technology.

Description

technical field [0001] The invention relates to a method for micro-cuttage transplantation in the field of plant regeneration technology, in particular to a method for rapidly producing Chinese fir seedlings by micro-cuttage, and belongs to the field of plant biotechnology. Background technique [0002] Chinese fir is a unique native tree species in my country and one of the most important commercial timber tree species in the south of the Yangtze River. It has the advantages of fast growth, high yield, good material and wide application. It is widely used in Guizhou, Hunan, Guangxi, Guangdong, Fujian and Jiangxi. Widely cultivated in other provinces. As one of the central producing areas of Chinese fir, Guangxi has a good provenance reserve, but its cultivation method is based on seedling afforestation for seed propagation. Due to the influence of climate and other factors, the output of Chinese fir seed gardens fluctuates greatly, often facing the problem of seedlings. lac...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G9/10A01G1/00
Inventor 杨梅徐圆圆杨家鸿黄寿先戴勤卢亮蒋维昕周传明
Owner GUANGXI UNIV
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