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Extraction method of bean dreg protein and method used for preparing DPP-IV inhibitory peptide with bean dreg protein

A DPP-IV and extraction method technology is applied in the field of preparation of DPP-IV inhibitory peptides and bean dregs protein extraction, and can solve the problems of high price, cumbersome procedures, poor product stability, etc., and achieves avoiding pollution, simple process, and no toxic and side effects. Effect

Active Publication Date: 2015-01-07
INST AGRO PROD PROCESSING ANHUI ACADEMY AGRI SCI
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Problems solved by technology

In addition, Chinese patent CN101029081 discloses that the recombinant protein Exendin-4 polypeptide has DPP-IV inhibitory properties. The polypeptide is usually produced by chemical synthesis and genetically engineered bacteria fermentation. The process is cumbersome, the price is relatively expensive, and the product is stable. also poor

Method used

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  • Extraction method of bean dreg protein and method used for preparing DPP-IV inhibitory peptide with bean dreg protein

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Embodiment 1

[0038] 1. Extraction method of okara protein

[0039] Mix 10g of fresh bean dregs with 500ml of water evenly, place the bean dregs suspension in a constant temperature box at 35°C, measure the pH value to 4.5 after 8 hours, take it out, cool it at room temperature, take out the upper floating layer, and use 12 Filter with a layer of gauze. After the filtration is completed, collect the remaining bean dregs protein on the gauze, and dry it naturally at room temperature to obtain 5.83g of protein with a moisture content of 12.31%.

[0040] Two, adopt the above-mentioned okara protein to prepare the method for DPP-IV inhibitory peptide

[0041]Take 5g of okara protein, mix it with 100ml of water, adjust the pH value to 9.0, add 0.05g of alkaline protease for enzymolysis, maintain the pH value of 9.0±0.5 during the enzymolysis process, maintain the temperature at 20-60°C, and carry out the hydrolysis reaction for 6 hours Finally, use boiling water to inactivate the enzyme for 10 ...

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Abstract

The invention relates to an extraction method of bean dreg protein and a method used for preparing DPP-IV inhibitory peptide with the bean dreg protein. The extraction method comprises the following steps: placing bean dreg suspension mixed by fresh bean dregs and water at the temperature of 30-50 DEG C, taking out the bean dreg suspension when pH is 3.0-5.0, collecting the lowermost layer of bean dreg protein after the suspension is layered, airing the bean dreg protein at room temperature, adding protease to prepare DPP-IV inhibitory peptide crude product powder, and then carrying out membrane separation or column chromatography to further purify to obtain DPP-IV inhibitory peptide pure product. The extraction method of the bean dreg protein is simple, no acid liquor or alkali liquor needs to be added in the preparation process, and pollution on the environment is avoided. For the method for preparing the DPP-IV inhibitory peptide with the bean dreg protein, the process is simple, the cost is low, the inhibitory ratio of the prepared DPP-IV inhibitory peptide is between 4.1% and 93.2%, and the median inhibitory concentration is between 42.70 mug / mL and 2593.64 mug / mL.

Description

technical field [0001] The invention belongs to the field of health food, and in particular relates to a method for extracting bean dregs protein and a method for preparing DPP-IV inhibitory peptide. Background technique [0002] During the processing of tofu, soymilk and soybean protein isolate (SPI), a large amount of by-product okara is produced, and okara contains 15% to 20% protein. Domestically, about 4 million tons of soybeans are used to process tofu or soy milk, and about 3 million tons of soybeans are used to process soybean protein isolates. The amount of soybean dregs produced is very large. Bean dregs is a kind of fibrous residue. For a long time, due to the high water content of fresh bean dregs (up to 75-78%), it is easy to spoil and deteriorate. It is usually discarded as waste and rarely used for food. fertilizer. Therefore, the utilization rate of bean dregs is low, and its potential nutritional value has not been fully developed and utilized, which is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14C12P21/06
Inventor 谢宁宁黄晶晶程江华王灼琛余丽王晓莉王苗苗李迪龙赵友成殷俊峰闫晓明
Owner INST AGRO PROD PROCESSING ANHUI ACADEMY AGRI SCI
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