Novel feed additive

A feed additive, a new type of technology, applied in animal feed, animal feed, application, etc., can solve the problem of single species, achieve the effects of reducing application, protecting and improving the ecological environment, and reducing the incidence of intestinal diseases

Inactive Publication Date: 2015-01-21
SHENYANG TIANJIAHUA BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the disadvantages of single microbial preparations in the initial stage of development and few examples of cross-combination with other fields in the prior art, the present invention provides a new type of microbial preparation prepared by scientific compatibility after screening compound microecological preparations, sodium butyr...

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Experimental program
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Effect test

Embodiment 1

[0043] 1. Screening and preparation method of compound probiotics

[0044] (1) Screening of Bacillus subtilis

[0045] Under sterile conditions, a number of commercially available Bacillus subtilis (Chaoyang Huaxing Biological Engineering Co., Ltd.: total number of viable bacteria 2 × 10 10 cfu / g) were screened for resistance to 0.3% pig bile salt and PH=3 acid to obtain the optimal strain, inoculated into 50mL LB liquid medium respectively, cultured with shaking at 220rpm at 37±1°C, and cultured at 0h, 2h, 4h, 6h, 8h, 10h, 12h, 14h, 16h, 18h, 20h, 22h, 24h, 26h, 28h, 30h, 32h, 34h, 36h, 38h, 40h, the OD value was measured at an absorbance of 600nm to obtain Bacillus subtilis The growth curve, the result is as follows figure 1 As shown, Bacillus subtilis from the late exponential growth period to the late stationary phase were inoculated into LB medium, and cultured at 37±1°C with shaking at 220rpm for 12±2h;

[0046] (2) Cultivation of Lactobacillus casei

[0047] Under ...

Embodiment 2

[0058] In the present embodiment, preparation method is identical with embodiment 1, comprises the following steps:

[0059] 1. Screening and preparation method of compound probiotics

[0060] (1) Screening of Bacillus subtilis

[0061] Bacillus subtilis (Beijing Xindimei Feed Technology Co., Ltd.: the total number of viable bacteria 2 × 10 10 cfu / g) After screening the optimal strains resistant to 0.3% pig bile salt and PH=3 acid, they were inoculated into 50mL LB liquid medium respectively, cultured with shaking at 220rpm at 37±1°C, and taken from the late stage of exponential growth to stable The Bacillus subtilis in the late stage were inoculated into LB medium respectively, and cultured with shaking at 220rpm at 37±1°C for 12h;

[0062] (2) Cultivation of Lactobacillus casei

[0063] Lactobacillus casei (Beijing Xindimei Feed Technology Co., Ltd.: the total number of viable bacteria 2 × 10 10 cfu / g) After selecting the optimal strain resistant to 0.3% pig bile salt an...

Embodiment 3

[0074] In the present embodiment, preparation method is identical with embodiment 1, comprises the following steps:

[0075] 1. Screening and preparation method of compound probiotics

[0076] (1) Screening of Bacillus subtilis

[0077] Bacillus subtilis (Beijing Xindimei Feed Technology Co., Ltd.: 2 × 10 10 cfu / g) After screening the optimal strains resistant to 0.3% pig bile salt and PH=3 acid, they were inoculated into 50mL LB liquid medium respectively, cultured with shaking at 220rpm at 37±1°C, and taken from the late stage of exponential growth to stable Bacillus subtilis and Bacillus subtilis in the late stage were inoculated into LB medium respectively, and cultured with shaking at 220rpm at 37±1°C for 14h;

[0078] (2) Cultivation of Lactobacillus casei

[0079] Lactobacillus casei (Chaoyang Huaxing Biological Engineering Co., Ltd.: 2 × 10 10 cfu / g) After selecting the optimal strain resistant to 0.3% pig bile salt and resistant to PH=3 acid, inoculate it into 100...

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Abstract

The invention discloses a novel feed additive. The additive is composed of three main ingredients, namely a compound Chinese herbal medicine, a compound micro-ecological preparation and sodium butyrate, wherein the novel feed additive is prepared by mixing the compound Chinese herbal medicine, the compound micro-ecological preparation and sodium butyrate in a weight ratio of 1 to (1-2) to (2-3). Compared to the prior art, the feed additive disclosed by the invention has the advantages that by scientifically combining the compound micro-ecological preparation, chemical medicine and compound Chinese herbal medicine which supplement each other, the feed additive gives full play to a synergistic effect of the three ingredients, so that the feed additive can regulate the intestinal micro-ecological environment of a pig, effectively reduce intestinal diseases of the pig, improve growth performance and immunity of the pig, improve a culture environment and promote healthy and sustainable development of pig industry.

Description

technical field [0001] The invention relates to the field of feed additives and microecological preparations, in particular to a novel feed additive based on a compound probiotic preparation compatible with sodium butyrate and compound Chinese herbal medicines. Background technique [0002] In recent years, with the widespread use of feed antibiotic additives in the livestock and poultry industry, its disadvantages have become increasingly prominent. For example, the abuse of antibiotics has led to the imbalance of normal intestinal flora in animals, and the emergence of a large number of drug-resistant strains; Toxic and side effects; endangering public health safety; reducing the competitiveness of livestock and poultry products in the market; affecting the healthy development of animal husbandry and food safety and other prominent problems. Therefore, many countries have successively banned or restricted the application of antibiotics in feed. In view of the current situa...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 朱天勇
Owner SHENYANG TIANJIAHUA BIOTECH CO LTD
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