Application of dragon blood in preparation of medicine for preventing and treating autoimmune disease

An autoimmune, dragon's blood technology, applied in the field of medicine, can solve the problem of weakening the patient's immune defense response

Active Publication Date: 2015-01-28
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the traditional treatment of autoimmune diseases, the immunosuppressants (a...

Method used

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  • Application of dragon blood in preparation of medicine for preventing and treating autoimmune disease
  • Application of dragon blood in preparation of medicine for preventing and treating autoimmune disease
  • Application of dragon blood in preparation of medicine for preventing and treating autoimmune disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Inhibitory effect of Dracaena japonicus on mammalian Kv1.3 channel function

[0019] Jurkat E6-1T cells (ATCC TIB152) and HEK293T cells (ATCC ACS4500) were cultured in RPMI1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum, 100 units / ml penicillin, and 100 μg / ml streptomycin, respectively. and DMEM medium (Life Technologies, Grand Island, NY, USA), and the cells were cultured in an incubator at 37°C and 5% CO2. The cDNA encoding mKv1.3 channel protein in vector pSP64 was subcloned into pIRES2-EGFP (Clontech, Inc., Mountain View, CA, USA) vector via XhoI / BamHI multiple cloning site. The constructed clones were analyzed by DNA sequencing to confirm the correctness of their nucleotide sequences. The constructed vector was transfected into HEK293T cells with Lipofectamine2000 (Invitrogen), and used for electrophysiological experiments 24 hours later. The extracellular fluid components (mmol / L) used to record the Kv1.3 channel c...

Embodiment 2

[0027] Example 2 Inhibition of Dracaena japonicus on the release of inflammatory factor IL-2 in Jurktat cells

[0028]The opening of the Kv1.3 channel on the Jurkat T cell membrane causes the repolarization of the cell membrane potential, which is the main potential energy to maintain the continuous influx of extracellular calcium ions. Blocking the Kv1.3 channel current on the Jurkat T cell membrane will correspondingly prevent the influx of extracellular calcium ions, thereby reducing the concentration of intracellular free calcium ions. The cytokine IL-2 secreted by Jurkat T cells is affected by the intracellular free calcium concentration, and a decrease in the intracellular free calcium concentration will attenuate the release of the inflammatory cytokine IL-2. Since Dracaena can inhibit the Kv1.3 channel function of Jurkat T cells and cause cell membrane potential depolarization, this study intends to observe whether Dracaena can inhibit the release of inflammatory cytok...

Embodiment 3

[0031] Example 3 Therapeutic effect of dragon's blood jerky on autoimmune disease model animals

[0032] 1. Production of autoimmune encephalitis model

[0033] The experimental animals were Kunming male mice aged 6-8 weeks. After emulsifying 100 μg of myelin oligodendrocyte glycoprotein MOG35-55 (Sigma Company) with 100 μl of Complete Freund's Adjuvant (CFA), the mouse was immunized by subcutaneous injection on both sides of the armpit At the same time, 125ng of pertussis toxin (pertussis toxin, Sigma company) was injected intraperitoneally, and 125ng of pertussis toxin was injected intraperitoneally again 48 hours after inoculation. Mice were monitored daily for signs of clinical EAE and given a clinical score based on the following criteria: 0, no disease; 1, limp and drooping tail; 2, hindlimb weakness; 3, hindlimb paralysis; 4, moribund; 5, dead.

[0034] Experimental autoimmune encephalomyelitis (EAE) mice were randomly divided into three groups: normal control group (...

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Abstract

The invention discloses application of dragon blood in preparation of a medicine for preventing and treating autoimmune disease. According to the application, the effect of treating autoimmune disease is generated by inhibiting a Kv1.3 channel function, the medicine containing dragon blood is prepared into granules, tablets, hard capsules, oral solutions, soft capsules or drop pills. The treatment effect of dragon blood on treating autoimmune disease is disclosed, the side effect of adrenal cortex hormone and other traditional treatment medicines can be greatly overcome, and normal protective immune response of patients can be maintained.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of dragon's blood as a medicine for preventing and treating autoimmune diseases. Background technique [0002] 5-8% of the world's population is seriously threatened by about 40 autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. The immunosuppressants (adrenal cortex hormones, etc.) immune defense response. How to find new drug therapeutic targets, so that the target drugs can maintain the normal immune response of the body while treating autoimmune diseases, has become a new research and development hotspot in the international pharmaceutical industry. Dragon's blood is a traditional precious traditional Chinese medicine. Previous studies have shown that dragon's blood has the effect of inhibiting the proliferation of activated T lymphocytes, thereby producing immunosuppressive effects, suggesting the potential of dra...

Claims

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Application Information

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IPC IPC(8): A61K36/896A61P37/06
CPCA61K36/896
Inventor 尹世金胡青兰陆春兰李羽欣
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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