Method for producing nosiheptide employing fermentation of streptomyces actuosus

A technology of active Streptomyces and nosiheptide, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of low yield and high production cost, and achieve improved biological activity, improved yield, and guaranteed Effects on normal production and metabolism

Inactive Publication Date: 2015-03-04
SOOCHOW KH BIO SCI & TECH CO LTD KHB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing microbial fermentation method produces nosiheptide, which has high production cost and low yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The source of active Streptomyces used in this example is commercially available.

[0019] Prepare media according to the following recipes: slant media, seed pot media, and fermenter media.

[0020] Slant medium: soluble starch 2.1g / 100mL, K 2 HPO 4 0.08g / 100mL, MgSO 4 0.06g / 100mL, FeSO 4 0.003g / 100mL, NaCl0.03g / 100mL, KNO 3 0.02g / 100mL, agar 2.0g / 100mL;

[0021] Seed tank medium: glucose 2g / 100mL, soybean cake powder 2.5g / 100mL, (NH 4 ) 2 SO 4 0.2g / 100mL, MgSO 4 0.03g / 100mL, calcium carbonate CaCO 3 0.4g / 100mL, K 2 HPO 4 0.02g / 100mL, soybean oil 0.3g / 100mL, defoamer 0.02g / 100mL;

[0022] Fermentation tank medium: soluble starch 6.0g / 100mL, soybean cake powder 3.5g / 100mL, (NH 4 ) 2 SO 4 1.0g / 100mL, CaCO 3 1.0g / 100mL, K 2 HPO 4 0.08g / 100mL, NaCl 0.10g / 100mL, soybean oil 0.8g / 100mL, defoamer 0.05g / 100mL.

[0023] Use a sterile inoculation needle to dig an appropriate amount of sand and inoculate it on the slant medium for 8 days, add sterile water to t...

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Abstract

The invention discloses a method for producing nosiheptide employing fermentation of streptomyces actuosus. The method comprises the following three steps: carrying out spore cultivation on a slant culture-medium; carrying out bacterial culture on a seed tank culture medium; and fermenting on a fermentation tank culture medium, wherein culture or fermentation parameters of various steps are optimized. Compared with the prior art, the activation of the streptomyces actuosus is optimized; the biological activity is improved through cultivation of the streptomyces actuosus at different stages; and the yield of the nosiheptide is increased by over 60%.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to a method for fermenting and producing nosiheptide by active streptomyces. Background technique [0002] Nosiheptide is a polypeptide antibiotic with 5 wah rings. Nosiheptide is a yellow-green substance in the form of powder or fine needle crystals, with a melting point of 310-320°C (with decomposition). Nosiheptide is soluble in chloroform, pyridine, dimethylamine, dimethylsulfoxide, slightly soluble in methanol, ethanol, ethyl acetate and benzene, and insoluble in water. With its relative safety and good application effect, nosiheptide meets the current people's requirements for feed additives, and is an ideal substitute for feed antibiotics. Currently, it has been approved and widely used in the European Union and Japan. [0003] Nosiheptide is produced by microbial culture. Nosiheptide-producing bacteria mainly include active streptomyces (streptomyes actuosus), antibiotic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/465
Inventor 金京勋
Owner SOOCHOW KH BIO SCI & TECH CO LTD KHB
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