Unlock instant, AI-driven research and patent intelligence for your innovation.

Inactivation of a production strain using a fatty acid

A fatty acid, fatty acid salt technology, applied in the field of one or more desired enzymes

Inactive Publication Date: 2015-03-11
NOVOZYMES AS
View PDF15 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These enzymes can only be sold at relatively low prices

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inactivation of a production strain using a fatty acid
  • Inactivation of a production strain using a fatty acid
  • Inactivation of a production strain using a fatty acid

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0123] Production of cellulase in Trichoderma reesei followed by inactivation of the producing strain

[0124] Fermentation was carried out using Trichoderma reesei as the microbial host cell. Trichoderma reesei strains producing cellulases are publicly available, for example from DSMZ.

[0125] Frozen glycerol stocks were used as the inoculum to inoculate the flasks. Inoculated flasks were grown as indicated in the table below. During inoculation, 10% of the main tank solvent (approximately 10,000 liters) was used.

[0126] Trace metal preparation

[0127] components

g / L

FeCl 3 ·6H 2 0

216

ZnSO 4 ·7H 2 o

58

MnSO 4 ·H 2 o

27

CuSO 4 ·5H 2 o

10

[0128] Inoculation Substrate Mix

[0129] components

g / kg

Glucose Syrup (73% w / w)

27

corn syrup

19

(NH 4 ) 2 SO 4

1.5

K H 2 PO 4

2.1

CaCO 3

0.2

MgSO 4 ·7H 2 o ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
Login to View More

Abstract

A method of inactivating the microbial host cell in a fermentation broth comprising an enzyme of interest and the microbial host cell producing the enzyme of interest comprising: a) Adding a fatty acid having a chain length of C4-C12 to the fermentation broth; and b) Mixing the fermentation broth for a sufficient period of time until the microbial host cell is inactivated.

Description

technical field [0001] The present invention relates to a process for the production of a desired enzyme or enzymes as a crude product. Background technique [0002] Currently, microbial host cells are widely used to produce enzymes by fermentation. Enzymes are in great demand, especially in the field of biofuels for industrial use, such as, for example, cellulases for the conversion of plant material into syrups and / or fermentation products. These enzymes can only be sold at relatively low prices. This makes enzyme production cost an important factor for success in the market. [0003] One way to solve this problem is to produce a crude product, meaning that the microbial host cells in the fermentation broth have been inactivated, but recovery steps such as centrifugation and / or filtration have not been performed. Summary of the invention [0004] The inventors have discovered that microbial host cells can be inactivated by the use of fatty acids, we therefore claim: ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P21/02C12N1/00A01N37/02C12N1/14
CPCC12N9/2434C12N1/005A01N37/02C12N9/2437
Inventor H·M·延森M·埃勒高S·雅各布森
Owner NOVOZYMES AS