Transformed human acidic fibroblast growth factor gene and its recombinant vector and application

A fibroblast and growth factor technology, applied in the field of genetic engineering, can solve problems affecting application, environmental pollution, and increased production costs, and achieve the effect of promoting cell proliferation and reducing production costs

Active Publication Date: 2017-06-06
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of these new silks requires the use of acid, alkali, high temperature and other conditions to remove the sericin components in the silk in advance, which will increase production costs and cause environmental pollution, and affect its practical application

Method used

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  • Transformed human acidic fibroblast growth factor gene and its recombinant vector and application
  • Transformed human acidic fibroblast growth factor gene and its recombinant vector and application
  • Transformed human acidic fibroblast growth factor gene and its recombinant vector and application

Examples

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Embodiment Construction

[0022] Hereinafter, the preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental methods without specific conditions in the examples usually follow conventional conditions, such as the conditions described in the Molecular Cloning Experimental Guide (third edition, J. Sambrook et al.), or the conditions recommended by the manufacturer.

[0023] The cell line used in the examples is mouse fibroblast NIH / 3T3, which is preserved by the State Key Laboratory of Silkworm Genomic Biology, Southwest University, China.

[0024] 1. Preparation of silkworm with human acidic fibroblast growth factor (hFGF1) gene

[0025] The codon-optimized human acidic fibroblast growth factor (hFGF1) gene, that is, the modified human acidic fibroblast growth factor gene, will be synthesized commercially. Its nucleotide sequence is shown in SEQ ID NO.1. The upstream of the nucleotide shown in SEQ ID NO.1 is the BamHI restriction si...

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Abstract

A modified human acidic fibroblast growth factor gene is disclosed. The nucleotide sequence of the gene is shown as the number 25 locus to the number 450 locus of SEQ ID NO.1. The sequence is designed according to codon preferences of bombyx mori, and can be normally expressed and translated in the bombyx mori. A recombinant vector containing the modified human acidic fibroblast growth factor gene is also disclosed. The recombinant vector comprises an enhanser hr3 and a secreting type sericin 1 promoter Ser1, specifically expresses a modified human acidic fibroblast growth factor in a middle silk gland cell of the bombyx mori, and secretes a recombinant protein to a sericin layer of silk. The obtained silk has bioactivity, has a function of promoting cell proliferation when the silk is directly used, and is a good medical material.

Description

Technical field [0001] The invention belongs to the field of genetic engineering, and particularly relates to a modified human acidic fibroblast growth factor gene, and also relates to a recombinant vector containing the modified human acidic fibroblast growth factor gene and the application of the modified human acidic fibroblast growth factor gene. Background technique [0002] Bombyx mori is known for its silk secretion, and is one of the earliest economic animals (insects) fully domesticated and utilized by humans. The silk gland of the silkworm is an organ that synthesizes and secretes silk protein and is the biological basis of the entire silk industry. After thousands of years of artificial domestication, the silk glands of the silkworm have superb protein synthesis and secretion capabilities. A silkworm weighing about 5g can synthesize and secrete about 0.5g silk protein, which is the most known insect. Silk fibroin is mainly composed of fibroin and sericin wrapped in it...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N15/12C12N15/85A01K67/04A61L27/22
Inventor夏庆友王峰徐汉福王元成袁林马三垣赵萍
OwnerSOUTHWEST UNIV