Application of genipin in prevention or treatment of ischemic brain injury

A technology for ischemic brain injury and ischemic stroke, applied in the field of preparation of drugs for preventing and/or treating ischemic brain injury

Inactive Publication Date: 2015-04-22
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of genipin in prevention or treatment of ischemic brain injury
  • Application of genipin in prevention or treatment of ischemic brain injury
  • Application of genipin in prevention or treatment of ischemic brain injury

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0027] Experimental example 1 Protective effect of genipin on lipopolysaccharide (LPS)-induced BV2 cell damage

[0028] 1. Materials

[0029] 1.1 Source of cells

[0030] BV2 cells were purchased from Peking Union Medical College Cell Resource Center.

[0031] 1.2 Drugs and reagents

[0032] Genipin was purchased from Guangxi Shanyun Biochemical Technology Co., Ltd.; DMEM medium, glutamine, and double antibodies were purchased from GIBCO; CCK-8 kit was purchased from Shanghai Beibo Bio; tumor necrosis factor-α, interleukin-1- βELISA kits were purchased from Cloud clone corp, USA.

[0033] 1.3 Test equipment

[0034] Microplate reader (Reber MK3, Finland), inverted microscope (Olympus IX2-SLP, Olympus, Japan), ultra-low temperature centrifuge (Thermo, USA), carbon dioxide incubator (STIK, USA), ultra-clean Workbench (HFsafe-1500 biological safety cabinet, Haier, China), digital camera (Leica DC300, Germany), ultraviolet spectrophotometer (Shimadzu UV-2450, Japan).

[0035] ...

experiment example 2

[0062] Experimental example 2 Genipin to H 2 o 2 Protective effect on human umbilical vein endothelial cells (HUVEC) injury

[0063] 1. Materials

[0064] 1.1 Source of cells

[0065] HUVEC cells were purchased from Peking Union Medical College Cell Resource Center.

[0066] 1.2 Drugs and reagents

[0067] Genipin was purchased from Guangxi Shanyun Biochemical Technology Co., Ltd.; DMEM medium, glutamine, and double antibodies were purchased from GIBCO; CCK-8 kit was purchased from Shanghai Beibo Bio; Nitric oxide (NO) kit was purchased from From Nanjing Jiancheng Institute of Bioengineering; endothelial nitric oxide synthase (eNOS) kit and intercellular adhesion factor-1 (ICAM-1) kit were purchased from Cloud clone corp, USA.

[0068] 1.3 Test equipment

[0069] Microplate reader (Reber MK3, Finland), inverted microscope (Olympus IX2-SLP, Olympus, Japan), ultra-low temperature centrifuge (Thermo, USA), carbon dioxide incubator (STIK, USA), ultra-clean Workbench (HFsafe...

experiment example 3

[0098] Experimental example 3 Genipin anti-cerebral ischemia experimental research

[0099] The use of mice to make cerebral ischemia models is the most commonly used method for anti-ischemia research. Commonly used models include global cerebral ischemia, permanent focal cerebral ischemia and focal cerebral ischemia-reperfusion, each of which has its own characteristics. Angle mimics the pathogenesis of the acute phase of ischemic stroke. The present invention utilizes rat permanent cerebral ischemia model (pMCAO) to evaluate the curative effect of genipin anti-cerebral ischemia drug,

[0100] 1. Materials

[0101] 1.1 Experimental animals and sources

[0102] Healthy male Sprague-Dawley (SD) pathogen-free (SPF grade) rats, aged 7 months, weighing 250-270 g, were purchased from the Department of Laboratory Animal Science, Peking University Health Science Center (certificate number: SCXK (Beijing) 2011-0012) , raised in the Experimental Animal Center of the Institute of Chi...

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Abstract

The invention relates to application of genipin in prevention or treatment of ischemic brain injury. Brain injury is particularly related to injury of microglial cells or human umbilical vein endothelial cells. The invention further relates to a medicine composition containing genipin for preventing or treating ischemic brain injury.

Description

technical field [0001] The present invention relates to the use of genipin and the pharmaceutical composition containing it in the preparation of medicines for preventing and / or treating ischemic brain injury. Background technique [0002] Cerebrovascular disease is one of the three major diseases that lead to human death. Among them, ischemic cerebrovascular disease (ie, ischemic brain injury) is a common clinical critical disease, which refers to the local brain tissue including nerve cells, glial cells and other cells. Degeneration, necrosis or transient loss of function due to blood supply disorders account for about 80% of cerebrovascular diseases, and become a serious threat to human life and health due to its high morbidity, high mortality, high disability rate, and high recurrence rate. one of the main diseases. Ischemic brain injury is extremely harmful to the health of patients and often results in irreversible brain damage. Due to the interruption of cerebral ar...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P9/10A61P25/28A61P25/08A61P25/16
CPCA61K31/352
Inventor 杨洪军刘欣
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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