A kind of high-performance liquid chromatography determination method of free ibuprofen in arginoprofen

A technology of high-performance liquid chromatography and a determination method, which is applied in the field of high-performance liquid chromatography determination of free ibuprofen in arginoprofen, can solve the problems of low specificity and inaccuracy, reduce sample decomposition and ensure reliability performance, the effect of optimizing the conditions of HPLC

Active Publication Date: 2016-10-05
仁合熙德隆药业有限公司
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AI Technical Summary

Problems solved by technology

An important test item in the quality standard of argininoprofen is to determine the content of free ibuprofen in the product. Currently, there is no test method given in the Pharmacopoeia. The quality control of the product is carried out by each enterprise using its own internal control standards, generally UV-Vis Spectrophotometry, but it has the disadvantages of low specificity and inaccuracy

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  • A kind of high-performance liquid chromatography determination method of free ibuprofen in arginoprofen
  • A kind of high-performance liquid chromatography determination method of free ibuprofen in arginoprofen
  • A kind of high-performance liquid chromatography determination method of free ibuprofen in arginoprofen

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Embodiment

[0021] A high-performance liquid chromatography assay method for free ibuprofen in arginoprofen, carried out according to the following steps:

[0022] 1) HPLC conditions: HPLC, Shimadzu LC-20AT, HPLC conditions: C18 column (4.6mm×250mm, 5um), column temperature 30°C, UV detector, detection wavelength 220nm; flow Phase: methanol: water (containing 2% H3PO4, pH = 2.5) = 75:25, flow rate: 1.0ml / min

[0023] 2) Preparation of the test solution: take 1g arginoprofen sample, add 20ml of chloroform, extract with magnetic stirring for 10min, filter out the chloroform, repeat the extraction twice with 20ml of chloroform for the filter residue, combine the chloroform For the methane extract, the filtrate was evaporated to dryness in a water bath at 50°C under reduced pressure, the chloroform was recovered, the residue obtained after concentration was dissolved in methanol, and the volume was adjusted to 100ml as the test solution.

[0024] 3) preparation of reference substance solutio...

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Abstract

The invention discloses a method for measuring free ibuprofen in arginine ibuprofen by virtue of a high-performance liquid chromatography. The method mainly comprises the steps of extracting free ibuprofen in a sample by virtue of a fat soluble organic solvent, measuring by virtue of the high-performance liquid chromatography after the constant volume is achieved, and measuring the content of the free ibuprofen in the sample by virtue of an external standard method. The method has the advantages of being stable, accurate, reliable, good in repeatability and convenient to generalize and use.

Description

technical field [0001] The invention relates to a drug analysis method, in particular to a high performance liquid chromatography determination method for free ibuprofen in arginoprofen. Background technique [0002] Ibuprofen (Ibuprfen) is an alkyl phenylpropionic acid anti-inflammatory, antipyretic and analgesic drug with definite curative effect, small toxic and side effects, and high safety created by the British Boots company in 1967 and listed in 1969. However, it is almost insoluble in water. Ordinary oral tablets dissolve slowly in the gastrointestinal tract and are eliminated quickly in the body. When treating chronic diseases such as rheumatism and rheumatoid arthritis, long-term frequent high-dose medication is required, which can easily lead to gastrointestinal bleeding and certain damage to the kidneys, making some patients unable to continue treatment. In order to meet the clinical requirements, a variety of dosage forms designed by foreign countries have come...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 蒲含林朱义波相东方
Owner 仁合熙德隆药业有限公司
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