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A group of ssr markers for distinguishing millet varieties and its application

A millet and variety technology, applied in the field of SSR marking, can solve the problems of difficult identification of millet varieties, difficulty in accurately distinguishing differences, inaccuracy, etc.

Active Publication Date: 2016-08-17
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the performance of morphological characters is closely related to the field environment, it is difficult to accurately distinguish the differences between different millet varieties (genotypes) by traditional methods, and these characters are limited by the planting season and must be planted in the field. observed
This has just caused the difficulty and inaccuracy of millet variety identification

Method used

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  • A group of ssr markers for distinguishing millet varieties and its application
  • A group of ssr markers for distinguishing millet varieties and its application
  • A group of ssr markers for distinguishing millet varieties and its application

Examples

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Embodiment 1

[0044] Embodiment 1, distinguish the complete primer of millet variety and method thereof

[0045] 1. A complete set of primers for distinguishing millet varieties

[0046] The present invention constructs a set of primers (as shown in Table 1) containing 10 primer pairs, and different varieties of millet can be quickly distinguished through the primer set of the present invention.

[0047] Table 1. Sequences and technical parameters of primer sets

[0048]

[0049] 2. The method of distinguishing millet varieties with a complete set of primers

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PUM

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Abstract

The invention discloses a group of SSR markers for differentiating millet varieties as well as an application of the markers. The SSR markers for differentiating millet varieties consist of a prime pair 1, a prime pair 2, a prime pair 3, a prime pair 4, a prime pair 5, a prime pair 6, a prime pair 7, a prime pair 8, a prime pair 9 and a prime pair 10. Experiments verify that being verified in farm varieties and improved varieties originated from different regions in Chinese for many times, the complete primers can be used for accurately and effectively differentiating millet varieties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a group of SSR markers for distinguishing millet varieties and applications thereof. Background technique [0002] Millet (Setaria italica (L.) Beauv.), also known as millet and millet, belongs to Poaceae and the genus Setaria. It is the main food crop in northern my country and has rich nutritional value. Millet is an ancient crop originated in my country. It has been used as the main cultivated crop for thousands of years to cultivate the northern civilization of my country, and is known as the nurturing crop of the Chinese nation. Because of its strong drought resistance and high water use efficiency, millet is considered to be a strategic reserve crop to deal with the increasingly serious water shortage in the future, and it plays an irreplaceable role in the construction of sustainable ecological agriculture today. my country is the country of origin of millet and h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2535/131
Inventor 刁现民贾冠清智慧王春芳刘晓彤牛振刚柴杨
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI