Novel effects of veratrol estrogen receptor antagonist
A technology of estrogen receptors and antagonists, applied in the field of Chinese herbal veratrum extracts
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Embodiment 1
[0177] Crush the veratrum medicinal material into a coarse powder, add 6 times the amount of distilled water, and reflux extraction for 2-3 times, each time for 1 hour. According to the actual administration concentration, concentrate under reduced pressure or dilute to the corresponding concentration for in vivo and in vitro experimental research.
Embodiment 2
[0179] Crush the Veratrum medicinal material into coarse powder, add 6 times the amount of 60-80% ethanol, and reflux extraction for 2-3 times, each time for 1 hour. According to the actual administration concentration, concentrate under reduced pressure or dilute to the corresponding concentration for in vivo and in vitro experimental research.
Embodiment 3
[0181] Crush the veratrum medicinal material into coarse powder, add 2 times the amount of ammonia solution with a volume fraction of 28%, soak for 1 hour; then add 20 times the volume of chloroform-methanol (volume ratio: 4:1), and place in a 70°C water bath Add and reflux for 2 hours, filter, add a mixed solvent to wash the filter residue, collect the filtrate, concentrate under reduced pressure or dilute to the corresponding concentration, and use it for in vitro experimental research.
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