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Series cultivation and rapid collection method for marine microalgae

A marine microalgae and rapid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor sedimentation effect, incomplete precipitation, and long precipitation time, so as to achieve less pollution and better growth , good light transmittance effect

Inactive Publication Date: 2015-05-06
LINYI UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

These collection methods either consume a lot of power and cost a lot, or the precipitation effect is poor, the precipitation time is long and the precipitation is not complete

Method used

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  • Series cultivation and rapid collection method for marine microalgae
  • Series cultivation and rapid collection method for marine microalgae
  • Series cultivation and rapid collection method for marine microalgae

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Embodiment Construction

[0018] A method for serial culture and rapid collection of marine microalgae, the specific method is as follows:

[0019] Preparation of step 1 culture medium

[0020] Culture medium composition formula: NaHCO 3 1 serving, NH 4 NO 3 0.2 parts, 0.1 parts of urea, 0.5 parts of human urine, Na 2 SiO 3 0.005 part, 1 part of f / 2 trace element solution, 1 part of f / 2 vitamin solution, 0.2 part of calcium phosphate, 1 part of cow dung leachate, 0.8 part of soil leachate, 0.5 part of sea mud extract, NiSiO 4 ·6H 2 O 0.02 parts, NH 4 VO 3 0.001 part, Co(NO 3 ) 2 ·6H 2 O0.05 parts, Na 2 WO 2 2H 2 O 0.03 parts, boiled and cooled 1000 parts of seawater; put into a brown ground stopper reagent bottle, seal it, put it on a shaker and shake it for 15 minutes, put it in a refrigerator at 4°C for future use; wherein the f / 2 trace element solution formula (according to Prepare 1000ml solution (meter): ZnSO 4 4H 2 O 23 mg, MnCl 2 4H 2 O 178 mg, CuSO 4 ·5H 2 O 10 mg, FeC 6 h ...

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Abstract

The invention discloses a series cultivation and rapid collection method for marine microalgae, belonging to the field of aquaculture cultivation and solving the deficiencies that marine microalgae is originally cultivated by adopting a photobioreactor or an open cement pond, which is large in capital construction investment, complicated in technology, high in technical requirement, easy to pollution, troublesome to operate and low in efficiency. The sequential cultivation and rapid collection method for marine microalgae has the technical characteristics that the two microalgaes are mixedly bred so as to achieve mutual advantages and mutual promotion and reduce the resource waste; introduced gas can be utilized for three times by adopting the sequential cultivation of conical flasks, so that the energy can be saved and cannot be easily contaminated; after a compound precipitator is added, the microalgae liquid is stored in a dark environment at the low temperature of 5 DEG C, the collection efficiency is high, the nutrition is not lost, the operation is simple and the practicability is strong.

Description

[0001] Technical field The present invention belongs to the field of aquaculture, and in particular relates to a method for serial cultivation and rapid collection of marine microalgae. Background technique [0002] The invention discloses a method for the mixed series cultivation and fast collection of Pyramid subcardioid and Isochrysis globosa. Platymonas subcordiformis is a marine single-celled microalgae that occurs in large numbers in coastal ponds and calm harbors. It belongs to Chlorophyta, Chlorophyceae, Volvox, and Chlamydomonas. The algal body is compressed, and the front view of the cell is broadly ovoid, with a wider front end and a shallow depression in the middle, with 4 flagella protruding from the depression. There is a large cup-shaped green pigment body in the cell. There is a protein nucleus at the back end of the algae, and a red eye point near the nucleus. 11-16 microns long, 7-9 microns wide, and 3.5-5 microns thick. Asexual reproduction, when the envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王培磊
Owner LINYI UNIVERSITY
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