A method of stressing Haematococcus pluvialis to rapidly accumulate astaxanthin

Abstract

The invention discloses a method for rapidly accumulating astaxanthin by forcing haematococcus pluvialis, belonging to the field of aquaculture. Formerly, haematococcus pluvialis is cultured by virtue of an annular track type cement pit or a photobioreactor, so that astaxanthin is low in yield and easily polluted, the process is complex, the operation is troublesome, and the investment of capital construction and the cost are high. According to the method, a two-stage culture method is utilized; in the first stage, the culture is carried out in a conical flask, relatively low temperature and illumination intensity are set, so that the growth of algal cells is benefited, and the damage caused by highlight to the cells is avoided; in the second stage, the aeration culture is carried out by virtue of a white plastic bucket, so that the temperature and the illumination intensity are increased, the illumination time is prolonged, and the accumulation of astaxanthin is benefited. A formula of traditional culture liquid is further optimized, so that nutrients are relatively comprehensive and balanced, the cost is low, the operation is flexible and convenient, and the yield of astaxanthin is increased by 120%.

Description

technical field [0001] The invention belongs to the field of aquaculture, in particular to a method for stressing Haematococcus pluvialis to quickly accumulate astaxanthin. Background technique [0002] Haematococcus pluvialis, also known as Haematococcus pluvialis or Haematococcus lacustrine, widely exists in freshwater bodies rich in organic matter. It belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococcus, and Haematococcus. The content of astaxanthin in Haematococcus pluvialis is 1.5-3.0%, which is regarded as the concentrated product of natural astaxanthin. Astaxanthin is a highly efficient natural antioxidant, its main function is to scavenge free radicals and improve the body's anti-aging ability. A large number of studies have shown that the rate and yield of astaxanthin accumulated by Haematococcus pluvialis are higher than those of other organisms, and the ratio of astaxanthin and its esters contained in Haematococcus pluvialls (about 70% monoester, 25% die...

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Problems solved by technology

[0004] Regarding the method of coercing Haematococcus pluvialls to accumulate astaxanthin in large quantities, in the past, Yan Xiaojun disclosed a method for producing astaxanthin from Haematococcus pluvialls (application number 201310038104). Natural culture in the middle of 6-8 days, let it proliferate, then add potassium dihydrogen phosphate and sodium nitrate in the pool water, and cover the pool with a red film, under the irradiation of light waves with a wavelength of 630-760nm, let the cells of Haematococcus pluvialis Rapid growth to the transformed state; the transformed algae cells are in the pool water with a concentration of 0.02-0.05 g / L of potassium dihydrogen phosphate, and the pool is covered with a blue film. Under the irradiation of light waves with a wavelength of 430-490nm, the rain produces red The algae cells are cultivated to maturity to obtain Haematococcus pluvialis rich in astaxanthin; potassium dihydrogen phosphate, sodium nitrate and red light can make the algae cells grow rapidly and reach the transformation state in a short time; potassium dihydrogen phosphate, Blue light can make algal cells grow and mature quickly and accumulate astaxanthin; this method requires less investment, is simple to cultivate, and has high benefits, but it is troublesome to operate, easy to pollute, and difficult to popularize and apply in production

In addition, Xu Qingshan also invented a transformation method for cultivating Haematococcus pluvialis to produce astaxanthin (application number 201210561399), including the preparation of immobilized transformed cells, the combination of immobilized transformed cells and vegetative growth cells, and the related environment Condition control, etc., the steps are as follows: 1) prepare high-density Haematococcus pluvialis transformed cells; 2) prepare immobilized gel carrier; 3) prepare immobilized Haematococcus pluvialls transformed cell gel carrier; 4) as required concentration of algae species, add the Haematococcus pluvialis transformed cell gel carrier prepared in step 3, immobilize the gel carrier and the cultured cells to be transformed; 5) control the temperature at 24-26°C, control the light at 8000Lux, and adjust The water level method is to control the water level at 0.5m, and the method of flowing water is used to realize the induced transformation of immobilized transformed cells; the method provided by the present invention has obvious induction effect and stable and reliable operation, but the process is complicated, the operation is troublesome, the capital investment is large, and the cost is high , poor benefit

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